Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system

Wataru Mizunoya, Jun ichiro Wakamatsu, Ryuichi Tatsumi, Yoshihide Ikeuchi

研究成果: ジャーナルへの寄稿記事

71 引用 (Scopus)

抄録

Skeletal muscle comprises several fiber types classified based on their contractile and metabolic properties. Skeletal muscle fiber types are classified according to their myosin heavy chain isoforms (MyHC I, IIa, IIx, and IIb). We attained good separation of MyHC isoforms in a mini-gel system by modifying a previously developed electrophoresis protocol. Increased glycerol and decreased cross-linking agent concentrations improved the separation of MyHC isoforms. Sample preparation with dithiothreitol and protease inhibitors produced clear MyHC band boundaries. This protocol included silver staining, with a linear range. The protocol provided high resolution and a highly accurate assay of rodent MyHC isoforms.

元の言語英語
ページ(範囲)111-113
ページ数3
ジャーナルAnalytical Biochemistry
377
発行部数1
DOI
出版物ステータス出版済み - 6 1 2008

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Myosin Heavy Chains
Electrophoresis
Rodentia
Protein Isoforms
Gels
Muscle
Silver Staining
Fibers
Dithiothreitol
Skeletal Muscle Fibers
Protease Inhibitors
Silver
Glycerol
Assays
Skeletal Muscle

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system. / Mizunoya, Wataru; Wakamatsu, Jun ichiro; Tatsumi, Ryuichi; Ikeuchi, Yoshihide.

:: Analytical Biochemistry, 巻 377, 番号 1, 01.06.2008, p. 111-113.

研究成果: ジャーナルへの寄稿記事

Mizunoya, Wataru ; Wakamatsu, Jun ichiro ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide. / Protocol for high-resolution separation of rodent myosin heavy chain isoforms in a mini-gel electrophoresis system. :: Analytical Biochemistry. 2008 ; 巻 377, 番号 1. pp. 111-113.
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