PRX2 and PRX25, peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis

Joan Renard, Irene Martínez-Almonacid, Annika Sonntag, Isabel Molina, José Moya-Cuevas, Gaetano Bissoli, Jesús Muñoz-Bertomeu, Isabel Faus, Regina Niñoles, Jun Shigeto, Yuji Tsutsumi, José Gadea, Ramón Serrano, Eduardo Bueso

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抜粋

Permeability is a crucial trait that affects seed longevity and is regulated by different polymers including proanthocyanidins, suberin, cutin and lignin located in the seed coat. By testing mutants in suberin transport and biosynthesis, we demonstrate the importance of this biopolymer to cope with seed deterioration. Transcriptomic analysis of cog1-2D, a gain-of-function mutant with increased seed longevity, revealed the upregulation of several peroxidase genes. Reverse genetics analysing seed longevity uncovered redundancy within the seed coat peroxidase gene family; however, after controlled deterioration treatment, seeds from the prx2 prx25 double and prx2 prx25 prx71 triple mutant plants presented lower germination than wild-type plants. Transmission electron microscopy analysis of the seed coat of these mutants showed a thinner palisade layer, but no changes were observed in proanthocyanidin accumulation or in the cuticle layer. Spectrophotometric quantification of acetyl bromide-soluble lignin components indicated changes in the amount of total polyphenolics derived from suberin and/or lignin in the mutant seeds. Finally, the increased seed coat permeability to tetrazolium salts observed in the prx2 prx25 and prx2 prx25 prx71 mutant lines suggested that the lower permeability of the seed coats caused by altered polyphenolics is likely to be the main reason explaining their reduced seed longevity.

元の言語英語
ページ(範囲)315-326
ページ数12
ジャーナルPlant Cell and Environment
43
発行部数2
DOI
出版物ステータス出版済み - 2 1 2020

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

Renard, J., Martínez-Almonacid, I., Sonntag, A., Molina, I., Moya-Cuevas, J., Bissoli, G., ... Bueso, E. (2020). PRX2 and PRX25, peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis. Plant Cell and Environment, 43(2), 315-326. https://doi.org/10.1111/pce.13656