抄録
The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 861-868 |
| ページ数 | 8 |
| ジャーナル | Molecular Microbiology |
| 巻 | 3 |
| 発行部数 | 7 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1989 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
これを引用
Pseudomonas aeruginosa cytotoxin : the nucleotide sequence of the gene and the mechanism of activation of the protoxin. / Hayashi, Tetsuya; Kamio, Y.; Hishinuma, F.; Usami, Y.; Titani, K.; Terawaki, Y.
：: Molecular Microbiology, 巻 3, 番号 7, 01.01.1989, p. 861-868.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Pseudomonas aeruginosa cytotoxin
T2 - the nucleotide sequence of the gene and the mechanism of activation of the protoxin
AU - Hayashi, Tetsuya
AU - Kamio, Y.
AU - Hishinuma, F.
AU - Usami, Y.
AU - Titani, K.
AU - Terawaki, Y.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.
AB - The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024384809&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1989.tb00235.x
DO - 10.1111/j.1365-2958.1989.tb00235.x
M3 - Article
C2 - 2507866
AN - SCOPUS:0024384809
VL - 3
SP - 861
EP - 868
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 7
ER -