Pseudomonas aeruginosa cytotoxin: the nucleotide sequence of the gene and the mechanism of activation of the protoxin

Tetsuya Hayashi, Y. Kamio, F. Hishinuma, Y. Usami, K. Titani, Y. Terawaki

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.

元の言語英語
ページ(範囲)861-868
ページ数8
ジャーナルMolecular Microbiology
3
発行部数7
DOI
出版物ステータス出版済み - 1 1 1989
外部発表Yes

Fingerprint

Cytotoxins
Transcriptional Activation
Genes
Pseudomonas aeruginosa
Amino Acids
Trypsin
Pseudomonas aeruginosa Cytotoxins
Plasmids
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

Pseudomonas aeruginosa cytotoxin : the nucleotide sequence of the gene and the mechanism of activation of the protoxin. / Hayashi, Tetsuya; Kamio, Y.; Hishinuma, F.; Usami, Y.; Titani, K.; Terawaki, Y.

:: Molecular Microbiology, 巻 3, 番号 7, 01.01.1989, p. 861-868.

研究成果: ジャーナルへの寄稿記事

Hayashi, Tetsuya ; Kamio, Y. ; Hishinuma, F. ; Usami, Y. ; Titani, K. ; Terawaki, Y. / Pseudomonas aeruginosa cytotoxin : the nucleotide sequence of the gene and the mechanism of activation of the protoxin. :: Molecular Microbiology. 1989 ; 巻 3, 番号 7. pp. 861-868.
@article{db51675800f746deaf75be99abd617ef,
title = "Pseudomonas aeruginosa cytotoxin: the nucleotide sequence of the gene and the mechanism of activation of the protoxin",
abstract = "The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.",
author = "Tetsuya Hayashi and Y. Kamio and F. Hishinuma and Y. Usami and K. Titani and Y. Terawaki",
year = "1989",
month = "1",
day = "1",
doi = "10.1111/j.1365-2958.1989.tb00235.x",
language = "English",
volume = "3",
pages = "861--868",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Pseudomonas aeruginosa cytotoxin

T2 - the nucleotide sequence of the gene and the mechanism of activation of the protoxin

AU - Hayashi, Tetsuya

AU - Kamio, Y.

AU - Hishinuma, F.

AU - Usami, Y.

AU - Titani, K.

AU - Terawaki, Y.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.

AB - The gene encoding cytotoxin (ctx) was cloned from Pseudomonas aeruginosa 158 and the nucleotide sequence was determined. The structural gene of ctx encodes the procytotoxin of 286 amino acid residues with a molecular mass of 31681 Daltons. Procytotoxin was activated by removal of 20 amino acid residues from the C terminus with trypsin. The cloned ctx gene was not expressed in either an Escherichia coli strain or a cytotoxin non‐producing strain of P. aeruginosa. An expression system for the ctx gene was constructed by placing the structural gene of ctx downstream of tac promoter on a broad host‐range vector plasmid.

UR - http://www.scopus.com/inward/record.url?scp=0024384809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024384809&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.1989.tb00235.x

DO - 10.1111/j.1365-2958.1989.tb00235.x

M3 - Article

C2 - 2507866

AN - SCOPUS:0024384809

VL - 3

SP - 861

EP - 868

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 7

ER -