Pulsed EPR imaging of nitroxides in mice

Fuminori Hyodo, Shingo Matsumoto, Nallathamby Devasahayam, Christopher Dharmaraj, Sankaran Subramanian, James B. Mitchell, Murali C. Krishna

    研究成果: ジャーナルへの寄稿記事

    37 引用 (Scopus)

    抄録

    Nitroxides, unlike trityl radicals, have shorter T2s which until now were not detectable in vivo by a time-domain pulsed Electron Paramagnetic Resonance (EPR) spectrometer at 300 MHz since their phase memory times were shorter than the spectrometer recovery times. In the current version of the time-domain EPR spectrometer with improved spectrometer recovery times, the feasibility of detecting signals from nitroxide radicals was tested. Among the nitroxides evaluated, deuterated 15N-Tempone (15N-PDT) was found to have the longest T2. The signal intensity profile as a function of concentration of these agents was evaluated and a biphasic behavior was observed; beyond a nitroxide concentration of 1.5 mM, signal intensity was found to decrease as a result of self-broadening. Imaging experiments were carried out with 15N-PDT in solutions equilibrated with 0%, 5%, 10%, and 21% oxygen using the single point imaging (SPI) modality in EPR. The image intensity in these tubes was found to depend on the oxygen concentration which in turn influences the T2 of 15N-PDT. In vivo experiments were demonstrated with 15N-PDT in anesthetized mice where the distribution and metabolism of 15N-PDT could be monitored. This study, for the first time shows the capability to image a cell-permeable nitroxide in mice using pulsed EPR in the SPI modality.

    元の言語英語
    ページ(範囲)181-185
    ページ数5
    ジャーナルJournal of Magnetic Resonance
    197
    発行部数2
    DOI
    出版物ステータス出版済み - 4 1 2009

    Fingerprint

    Electron Spin Resonance Spectroscopy
    Paramagnetic resonance
    mice
    electron paramagnetic resonance
    Spectrometers
    Imaging techniques
    spectrometers
    Triacetoneamine-N-Oxyl
    recovery
    Oxygen
    Recovery
    oxygen
    metabolism
    Metabolism
    Experiments
    1-phenyl-3,3-dimethyltriazene
    tubes
    Data storage equipment
    profiles
    cells

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Nuclear and High Energy Physics
    • Condensed Matter Physics

    これを引用

    Hyodo, F., Matsumoto, S., Devasahayam, N., Dharmaraj, C., Subramanian, S., Mitchell, J. B., & Krishna, M. C. (2009). Pulsed EPR imaging of nitroxides in mice. Journal of Magnetic Resonance, 197(2), 181-185. https://doi.org/10.1016/j.jmr.2008.12.018

    Pulsed EPR imaging of nitroxides in mice. / Hyodo, Fuminori; Matsumoto, Shingo; Devasahayam, Nallathamby; Dharmaraj, Christopher; Subramanian, Sankaran; Mitchell, James B.; Krishna, Murali C.

    :: Journal of Magnetic Resonance, 巻 197, 番号 2, 01.04.2009, p. 181-185.

    研究成果: ジャーナルへの寄稿記事

    Hyodo, F, Matsumoto, S, Devasahayam, N, Dharmaraj, C, Subramanian, S, Mitchell, JB & Krishna, MC 2009, 'Pulsed EPR imaging of nitroxides in mice', Journal of Magnetic Resonance, 巻. 197, 番号 2, pp. 181-185. https://doi.org/10.1016/j.jmr.2008.12.018
    Hyodo F, Matsumoto S, Devasahayam N, Dharmaraj C, Subramanian S, Mitchell JB その他. Pulsed EPR imaging of nitroxides in mice. Journal of Magnetic Resonance. 2009 4 1;197(2):181-185. https://doi.org/10.1016/j.jmr.2008.12.018
    Hyodo, Fuminori ; Matsumoto, Shingo ; Devasahayam, Nallathamby ; Dharmaraj, Christopher ; Subramanian, Sankaran ; Mitchell, James B. ; Krishna, Murali C. / Pulsed EPR imaging of nitroxides in mice. :: Journal of Magnetic Resonance. 2009 ; 巻 197, 番号 2. pp. 181-185.
    @article{72fd54f5570a44f3af70d5756ae8ae78,
    title = "Pulsed EPR imaging of nitroxides in mice",
    abstract = "Nitroxides, unlike trityl radicals, have shorter T2s which until now were not detectable in vivo by a time-domain pulsed Electron Paramagnetic Resonance (EPR) spectrometer at 300 MHz since their phase memory times were shorter than the spectrometer recovery times. In the current version of the time-domain EPR spectrometer with improved spectrometer recovery times, the feasibility of detecting signals from nitroxide radicals was tested. Among the nitroxides evaluated, deuterated 15N-Tempone (15N-PDT) was found to have the longest T2. The signal intensity profile as a function of concentration of these agents was evaluated and a biphasic behavior was observed; beyond a nitroxide concentration of 1.5 mM, signal intensity was found to decrease as a result of self-broadening. Imaging experiments were carried out with 15N-PDT in solutions equilibrated with 0{\%}, 5{\%}, 10{\%}, and 21{\%} oxygen using the single point imaging (SPI) modality in EPR. The image intensity in these tubes was found to depend on the oxygen concentration which in turn influences the T2 of 15N-PDT. In vivo experiments were demonstrated with 15N-PDT in anesthetized mice where the distribution and metabolism of 15N-PDT could be monitored. This study, for the first time shows the capability to image a cell-permeable nitroxide in mice using pulsed EPR in the SPI modality.",
    author = "Fuminori Hyodo and Shingo Matsumoto and Nallathamby Devasahayam and Christopher Dharmaraj and Sankaran Subramanian and Mitchell, {James B.} and Krishna, {Murali C.}",
    year = "2009",
    month = "4",
    day = "1",
    doi = "10.1016/j.jmr.2008.12.018",
    language = "English",
    volume = "197",
    pages = "181--185",
    journal = "Journal of Magnetic Resonance",
    issn = "1090-7807",
    publisher = "Academic Press Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Pulsed EPR imaging of nitroxides in mice

    AU - Hyodo, Fuminori

    AU - Matsumoto, Shingo

    AU - Devasahayam, Nallathamby

    AU - Dharmaraj, Christopher

    AU - Subramanian, Sankaran

    AU - Mitchell, James B.

    AU - Krishna, Murali C.

    PY - 2009/4/1

    Y1 - 2009/4/1

    N2 - Nitroxides, unlike trityl radicals, have shorter T2s which until now were not detectable in vivo by a time-domain pulsed Electron Paramagnetic Resonance (EPR) spectrometer at 300 MHz since their phase memory times were shorter than the spectrometer recovery times. In the current version of the time-domain EPR spectrometer with improved spectrometer recovery times, the feasibility of detecting signals from nitroxide radicals was tested. Among the nitroxides evaluated, deuterated 15N-Tempone (15N-PDT) was found to have the longest T2. The signal intensity profile as a function of concentration of these agents was evaluated and a biphasic behavior was observed; beyond a nitroxide concentration of 1.5 mM, signal intensity was found to decrease as a result of self-broadening. Imaging experiments were carried out with 15N-PDT in solutions equilibrated with 0%, 5%, 10%, and 21% oxygen using the single point imaging (SPI) modality in EPR. The image intensity in these tubes was found to depend on the oxygen concentration which in turn influences the T2 of 15N-PDT. In vivo experiments were demonstrated with 15N-PDT in anesthetized mice where the distribution and metabolism of 15N-PDT could be monitored. This study, for the first time shows the capability to image a cell-permeable nitroxide in mice using pulsed EPR in the SPI modality.

    AB - Nitroxides, unlike trityl radicals, have shorter T2s which until now were not detectable in vivo by a time-domain pulsed Electron Paramagnetic Resonance (EPR) spectrometer at 300 MHz since their phase memory times were shorter than the spectrometer recovery times. In the current version of the time-domain EPR spectrometer with improved spectrometer recovery times, the feasibility of detecting signals from nitroxide radicals was tested. Among the nitroxides evaluated, deuterated 15N-Tempone (15N-PDT) was found to have the longest T2. The signal intensity profile as a function of concentration of these agents was evaluated and a biphasic behavior was observed; beyond a nitroxide concentration of 1.5 mM, signal intensity was found to decrease as a result of self-broadening. Imaging experiments were carried out with 15N-PDT in solutions equilibrated with 0%, 5%, 10%, and 21% oxygen using the single point imaging (SPI) modality in EPR. The image intensity in these tubes was found to depend on the oxygen concentration which in turn influences the T2 of 15N-PDT. In vivo experiments were demonstrated with 15N-PDT in anesthetized mice where the distribution and metabolism of 15N-PDT could be monitored. This study, for the first time shows the capability to image a cell-permeable nitroxide in mice using pulsed EPR in the SPI modality.

    UR - http://www.scopus.com/inward/record.url?scp=62549145710&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=62549145710&partnerID=8YFLogxK

    U2 - 10.1016/j.jmr.2008.12.018

    DO - 10.1016/j.jmr.2008.12.018

    M3 - Article

    C2 - 19157932

    AN - SCOPUS:62549145710

    VL - 197

    SP - 181

    EP - 185

    JO - Journal of Magnetic Resonance

    JF - Journal of Magnetic Resonance

    SN - 1090-7807

    IS - 2

    ER -