Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle

Noriyo Yoshii; Yoshitoshi Ogura; Tetsuya Hayashi; Takashi Ajiro; Toshiya Sameshima; Muneo Nakazawa; Masahiro Kusumoto; Taketoshi Iwata; Masato Akiba

doi:10.1128/AEM.00558-09

Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle

Noriyo Yoshii, Yoshitoshi Ogura, Tetsuya Hayashi, Takashi Ajiro, Toshiya Sameshima, Muneo Nakazawa, Masahiro Kusumoto, Taketoshi Iwata, Masato Akiba

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

15 被引用数 (Scopus)

抄録

A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

本文言語	英語
ページ（範囲）	5719-5726
ページ数	8
ジャーナル	Applied and environmental microbiology
巻	75
号	17
DOI	https://doi.org/10.1128/AEM.00558-09
出版ステータス	出版済み - 9月 2009
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
食品科学
応用微生物学とバイオテクノロジー
生態学

文献へのアクセス

10.1128/AEM.00558-09

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フィンガープリント

「Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Yoshii, N., Ogura, Y., Hayashi, T., Ajiro, T., Sameshima, T., Nakazawa, M., Kusumoto, M., Iwata, T., & Akiba, M. (2009). Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle. Applied and environmental microbiology, 75(17), 5719-5726. https://doi.org/10.1128/AEM.00558-09

Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle. / Yoshii, Noriyo; Ogura, Yoshitoshi; Hayashi, Tetsuya その他.

In: Applied and environmental microbiology, Vol. 75, No. 17, 09.2009, p. 5719-5726.

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

Yoshii, N, Ogura, Y, Hayashi, T, Ajiro, T, Sameshima, T, Nakazawa, M, Kusumoto, M, Iwata, T & Akiba, M 2009, 'Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle', Applied and environmental microbiology, vol. 75, no. 17, pp. 5719-5726. https://doi.org/10.1128/AEM.00558-09

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