Pulsed-field gel electrophoresis profile changes resulting from spontaneous chromosomal deletions in enterohemorrhagic Escherichia coli O157:H7 during passage in cattle

Noriyo Yoshii, Yoshitoshi Ogura, Tetsuya Hayashi, Takashi Ajiro, Toshiya Sameshima, Muneo Nakazawa, Masahiro Kusumoto, Taketoshi Iwata, Masato Akiba

研究成果: ジャーナルへの寄稿学術誌査読

15 被引用数 (Scopus)

抄録

A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

本文言語英語
ページ(範囲)5719-5726
ページ数8
ジャーナルApplied and environmental microbiology
75
17
DOI
出版ステータス出版済み - 9月 2009
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー
  • 生態学

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