Purification and Antigenic Analysis of Flagella of Campylobacter jejuni

Yuji Ueki, Shuji Fujimoto, Akiko Umeda, Kazunobu Amako

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The flagella of Campylobacter jejuni strain FUM158432 were purified and a flagellin preparation consisting of only a single peptide of 63,000 daltons was obtained. The peptide of 92,000 daltons usually associated with a flagellar preparation was shown to be a peptide derived from the hook region. Antiserum was prepared by immunizing a rabbit with the flagellin preparation. The reaction of the antiserum was found to be highly specific for the flagellar filament by immunoelectron microscopy and for flagellin peptide by the immunoblotting method. Seven-teen of 23 clinically isolated strains of C. jejuni reacted with this antiserum but the other six strains did not, indicating the existence of antigenic variation of the flagella of C. jejuni. The flagella of a few strains of C. coli also reacted with this antiserum.

元の言語英語
ページ(範囲)327-337
ページ数11
ジャーナルMICROBIOLOGY and IMMUNOLOGY
32
発行部数4
DOI
出版物ステータス出版済み - 1 1 1988

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Campylobacter jejuni
Flagella
Flagellin
Immune Sera
Peptides
Antigenic Variation
Immunoelectron Microscopy
Immunoblotting
Rabbits

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

これを引用

Purification and Antigenic Analysis of Flagella of Campylobacter jejuni. / Ueki, Yuji; Fujimoto, Shuji; Umeda, Akiko; Amako, Kazunobu.

:: MICROBIOLOGY and IMMUNOLOGY, 巻 32, 番号 4, 01.01.1988, p. 327-337.

研究成果: ジャーナルへの寄稿記事

Ueki, Yuji ; Fujimoto, Shuji ; Umeda, Akiko ; Amako, Kazunobu. / Purification and Antigenic Analysis of Flagella of Campylobacter jejuni. :: MICROBIOLOGY and IMMUNOLOGY. 1988 ; 巻 32, 番号 4. pp. 327-337.
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