Purification and assays of tachylectin-2

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

Tachylectin-2, a 27-kDa protein consisting of a five-bladed β-propeller structure, is purified by three steps of chromatography, including dextran sulfate-Sepharose CL-6B, CM-Sepharose CL-6B, and Mono S. Three isolectins of tachylectin-2 including tachylectin-2a, -2b, and -2c are purified. These isolectins exhibit hemagglutinating activity against human A-type erythrocytes in a Ca2+-independent manner with tachylectin-2b showing the highest activity. Tachylectin-2b specifically agglutinates Staphylococcus saprophyticus KD. The tachylectin-2b-mediated hemagglutination is inhibited in the presence of GlcNAc and GalNAc. The association constants for GlcNAc and GalNAc are Ka = 1.95 × 104 M−1 and Ka = 1.11 × 103 M−1, respectively. Ultracentrifugation analysis shows that tachylectin-2b is present in monomer form in solution.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ309-316
ページ数8
DOI
出版ステータス出版済み - 2020

出版物シリーズ

名前Methods in Molecular Biology
2132
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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