Purification and characterization of 17α,20β-dihydroxy-4-pregnen-3-one binding protein from plasma of rainbow trout, Oncorhynchus mykiss

Michiyasu Yoshikuni, Hisami Matsushita, Naoki Shibata, Yoshitaka Nagahama

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

A 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP) (the natural maturation-inducing hormone of salmonid fish) binding protein (DBP) was purified from rainbow trout plasma. It had an apparent molecular weight of 110 kDa on native PAGE and was composed of two subunits with molecular weights of 50 and 55 kDa on SDS-PAGE. Enzymatic digestion of sugar chains converted both subunits to a single peptide with a molecular weight of 42.5 kDa. Scatchard analysis of 17α,20β-DP binding to purified DBP showed the presence of a single binding site with a K(d) of 21 nM and B(max) of 5 nmol/mg of protein. The affinities of various steroids were estimated by the displacement of [3H]17α,20β-DP binding in the decreasing order of 17α,20β-DP, testosterone, 17α-hydroxyprogesterone, progesterone, estradiol-17β, 17α,20β,21-trihydroxy-4-pregnen-3-one, and cortisol. We conclude that DBP is a sex-hormone binding globulin in rainbow trout.

本文言語英語
ページ(範囲)189-196
ページ数8
ジャーナルGeneral and Comparative Endocrinology
96
2
DOI
出版ステータス出版済み - 11月 1994
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 動物科学および動物学
  • 内分泌学

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