Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides

Kaoru Takegawa, Shinya Yamaguchi, Satoshi Miki, Takayuki Jikibara, Shojiro Iwahara

研究成果: Contribution to journalArticle査読

抄録

A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures.

本文言語英語
ページ(範囲)1969-1975
ページ数7
ジャーナルAgricultural and Biological Chemistry
55
8
DOI
出版ステータス出版済み - 1 1 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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