Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1

Sachi Okamoto, Lindsay D. Eltis

研究成果: ジャーナルへの寄稿学術誌査読

44 被引用数 (Scopus)

抄録

The microbial degradation of nitriles is of interest for bioremediation and green chemistry. We demonstrated that the soil bacterium Rhodococcus sp. RHA1 utilizes a range of nitriles, including acetonitrile, as growth substrates. Proteomic analysis identified 13 proteins that were more abundant in acetonitrile-grown cells, including an aliphatic amidase and a protein with no known homologue. Purification of a nitrile hydratase (NHase) from acetonitrile-grown cells identified the unknown protein as the β subunit of a two-subunit NHase. Sequence analysis revealed that the genes encoding the amidase (anhC) and the NHase (anhAB) occur in a 12.8 kbp cluster located on plasmid pRHL2. The anh gene cluster also encodes an acetyl-CoA hydrolase, transcriptional regulators, a putative cobalt transporter and a protein of unknown function. Striking features of the NHase include the amino acid sequence identity (32%) and large size (63 and 56 kDa) of the α and β subunits, as well as the enzyme's metal ion content (one cobalt, two copper and one zinc). The enzyme possessed similar specificities for acetonitrile and propionitrile (kcat/Km∼7 mM-1 s -1) followed by acrylonitrile and butyronitrile. We propose that this acetonitrile hydratase (ANHase) represents the first member of a previously unknown class of NHases.

本文言語英語
ページ(範囲)828-838
ページ数11
ジャーナルMolecular Microbiology
65
3
DOI
出版ステータス出版済み - 8月 2007
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学

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