Purification and characterization of cannabichromenic acid synthase from Cannabis sativa

Satoshi Morimoto, Kengo Komatsu, Futoshi Taura, Yukihiro Shoyama

研究成果: Contribution to journalArticle査読

64 被引用数 (Scopus)

抄録

Cannabichromenic acid synthase was purified to apparent homogeneity by sequential column chromatography including DEAE-cellulose, phenyl-Sepharose CL-4B, and hydroxylapatite. The enzyme catalysed the oxidocyclization of cannabigerolic acid and cannabinerolic acid to cannabichromenic acid. The K(m) values for both substrates were in the same order of magnitude although the V(max) value for the former was higher than that for the latter. These results suggested that cannabichromenic acid is predominantly formed from cannabigerolic acid rather than cannabinerolic acid. The enzyme required neither molecular oxygen nor hydrogen peroxide, indicating that the cannabichromenic acid synthase reaction proceeds through direct dehydrogenation without hydroxylation.

本文言語英語
ページ(範囲)1525-1529
ページ数5
ジャーナルPhytochemistry
49
6
DOI
出版ステータス出版済み - 11 20 1998

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 植物科学
  • 園芸学

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