Purification and characterization of chymotrypsin inhibitor CI-3 from hemolymph of silkworm, Bombyx mori

Qiaoling Zhao, Ningjia He, Koji Shirai, Hiroshi Fujii, Yutaka Banno, Kohji Yamamoto

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The chymotrypsin inhibitor 3 (CI-3) whose expression is controlled by Ict-E gene on the 22nd linkage group was purified from the larval hemolymph of silkworm Bombyx mori by combination of a series of column chromatography and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). The molecular weight of CI-3 was 40 kDa and its isoelectric point was 5.5. The results of analysis on the protein properties revealed that the inhibitory activity of CI-3 against α-chymotrypsin was remarkably stable at pH 6.9-10.6, but lost 30% and 70% of the inhibitory activity at pH 5.9 and pH 11.9, respectively. CI-3 was quite stable at the temperature between 0°C to 50°C and lost inhibitory activity completely at the temperatures higher than 60°C. CI-3 showed strong inhibitory activity toward α-chymotrypsin and silkworm digestive juice (DJ) protease.

元の言語英語
ページ(範囲)93-99
ページ数7
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
49
発行部数1
出版物ステータス出版済み - 2 1 2004

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Bombyx
Hemolymph
Chymotrypsin
chymotrypsin
Bombyx mori
silkworms
hemolymph
Polyacrylamide Gel Electrophoresis
polyacrylamide gel electrophoresis
digestive juices
Temperature
Isoelectric Point
isoelectric point
linkage groups
Chromatography
chromatography
temperature
Peptide Hydrolases
proteinases
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Purification and characterization of chymotrypsin inhibitor CI-3 from hemolymph of silkworm, Bombyx mori. / Zhao, Qiaoling; He, Ningjia; Shirai, Koji; Fujii, Hiroshi; Banno, Yutaka; Yamamoto, Kohji.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 49, 番号 1, 01.02.2004, p. 93-99.

研究成果: ジャーナルへの寄稿記事

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AU - Zhao, Qiaoling

AU - He, Ningjia

AU - Shirai, Koji

AU - Fujii, Hiroshi

AU - Banno, Yutaka

AU - Yamamoto, Kohji

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