Purification and characterization of cocoonase from the silkworm Bombyx mori

Hisayoshi Fukumori, Satoshi Teshiba, Yuichi Shigeoka, Kohji Yamamoto, Yutaka Banno, Yoichi Aso

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Cocoonase (CCN) which facilitates the degradation of a cocoon is recognized as a trypsin-like serine protease. In this study, CCN from the silkworm Bombyx mori was purified and comprehensively characterized. Its activity was maximal at about pH 9.8. It was stable above pH 3.4 at 4°C and below 50°C at pH 7.5. CuSO4, FeSO4, and ZnSO4 showed inhibitory effects on CCN, but other salts improved activity. Typical trypsin inhibitors inhibited CCN, but the relative inhibitory activities were much lower than those against bovine trypsin. An extract of cocoon shells inhibited trypsin, but it was only slightly inhibitory against CCN. There were significant differences in catalytic efficiencies and substrate specificities as between CCN and bovine trypsin.

本文言語英語
ページ(範囲)202-211
ページ数10
ジャーナルBioscience, Biotechnology and Biochemistry
78
2
DOI
出版ステータス出版済み - 2014

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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