Purification and characterization of cocoonase from the silkworm Bombyx mori

Hisayoshi Fukumori, Satoshi Teshiba, Yuichi Shigeoka, Kohji Yamamoto, Yutaka Banno, Yoichi Aso

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Cocoonase (CCN) which facilitates the degradation of a cocoon is recognized as a trypsin-like serine protease. In this study, CCN from the silkworm Bombyx mori was purified and comprehensively characterized. Its activity was maximal at about pH 9.8. It was stable above pH 3.4 at 4°C and below 50°C at pH 7.5. CuSO4, FeSO4, and ZnSO4 showed inhibitory effects on CCN, but other salts improved activity. Typical trypsin inhibitors inhibited CCN, but the relative inhibitory activities were much lower than those against bovine trypsin. An extract of cocoon shells inhibited trypsin, but it was only slightly inhibitory against CCN. There were significant differences in catalytic efficiencies and substrate specificities as between CCN and bovine trypsin.

元の言語英語
ページ(範囲)202-211
ページ数10
ジャーナルBioscience, Biotechnology and Biochemistry
78
発行部数2
DOI
出版物ステータス出版済み - 1 1 2014

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Bombyx
Trypsin
Purification
Trypsin Inhibitors
Substrate Specificity
Salts
Degradation
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Purification and characterization of cocoonase from the silkworm Bombyx mori. / Fukumori, Hisayoshi; Teshiba, Satoshi; Shigeoka, Yuichi; Yamamoto, Kohji; Banno, Yutaka; Aso, Yoichi.

:: Bioscience, Biotechnology and Biochemistry, 巻 78, 番号 2, 01.01.2014, p. 202-211.

研究成果: ジャーナルへの寄稿記事

Fukumori, Hisayoshi ; Teshiba, Satoshi ; Shigeoka, Yuichi ; Yamamoto, Kohji ; Banno, Yutaka ; Aso, Yoichi. / Purification and characterization of cocoonase from the silkworm Bombyx mori. :: Bioscience, Biotechnology and Biochemistry. 2014 ; 巻 78, 番号 2. pp. 202-211.
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