Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus

Yumi Oba, Yohei Shimasaki, Yuji Oshima, Hina Satone, Takeshi Kitano, Miki Nakao, Shun-Ichiro Kawabata, Tsuneo Honjo

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

We used gel filtration chromatography, anion-exchange chromatography and polyacrylamide gel electrophoresis to purify tributyltin-binding protein type 2 (TBT-bp 2) from plasma of Japanese flounder (Paralichthys olivaceus) injected intraperitoneally with TBT (5.0mg/kg body weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the molecular mass of TBT-bp 2 was approximately 48kDa, and isoelectric focusing-polyacrylamide gel electrophoresis indicated that the isoelectric point was approximately 3.0. TBT-bp 2 contained 40% N-glycan. The complete cDNA nucleotide sequence and the genome sequence of TBT-bp 2 were determined by means of rapid amplification of cDNA ends of liver tissue of Japanese flounder and a genome-walking technique, respectively. The 216 amino acid sequence of TBT-bp 2 showed 47% identity to the sequences of puffer fish (Takifugu pardalis) saxitoxin- and tetrodotoxin-binding protein but only 27% similarity to the sequence of TBT-bp 1. Analysis of the motif sequence of the amino acid sequence and the structure of the gene encoding TBT-bp 2 suggested that this protein belongs to the lipocalin superfamily.

元の言語英語
ページ(範囲)229-238
ページ数10
ジャーナルJournal of biochemistry
142
発行部数2
DOI
出版物ステータス出版済み - 8 1 2007

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Flounder
Purification
Carrier Proteins
Plasmas
Electrophoresis
Polyacrylamide Gel Electrophoresis
Chromatography
Amino Acid Sequence
Complementary DNA
Genes
Takifugu
Saxitoxin
Tetraodontiformes
Genome
Lipocalins
Amino Acids
Gene encoding
Isoelectric Point
Isoelectric Focusing
Molecular mass

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. / Oba, Yumi; Shimasaki, Yohei; Oshima, Yuji; Satone, Hina; Kitano, Takeshi; Nakao, Miki; Kawabata, Shun-Ichiro; Honjo, Tsuneo.

:: Journal of biochemistry, 巻 142, 番号 2, 01.08.2007, p. 229-238.

研究成果: ジャーナルへの寄稿記事

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abstract = "We used gel filtration chromatography, anion-exchange chromatography and polyacrylamide gel electrophoresis to purify tributyltin-binding protein type 2 (TBT-bp 2) from plasma of Japanese flounder (Paralichthys olivaceus) injected intraperitoneally with TBT (5.0mg/kg body weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the molecular mass of TBT-bp 2 was approximately 48kDa, and isoelectric focusing-polyacrylamide gel electrophoresis indicated that the isoelectric point was approximately 3.0. TBT-bp 2 contained 40{\%} N-glycan. The complete cDNA nucleotide sequence and the genome sequence of TBT-bp 2 were determined by means of rapid amplification of cDNA ends of liver tissue of Japanese flounder and a genome-walking technique, respectively. The 216 amino acid sequence of TBT-bp 2 showed 47{\%} identity to the sequences of puffer fish (Takifugu pardalis) saxitoxin- and tetrodotoxin-binding protein but only 27{\%} similarity to the sequence of TBT-bp 1. Analysis of the motif sequence of the amino acid sequence and the structure of the gene encoding TBT-bp 2 suggested that this protein belongs to the lipocalin superfamily.",
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AU - Shimasaki, Yohei

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AU - Satone, Hina

AU - Kitano, Takeshi

AU - Nakao, Miki

AU - Kawabata, Shun-Ichiro

AU - Honjo, Tsuneo

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