Purification and characterization of tributyltin-binding protein of tiger puffer, Takifugu rubripes

Yumi Oba, Akira Yamauchi, Yasuyuki Hashiguchi, Hina Satone, Shizuho Miki, Mohamed Nassef, Yohei Shimasaki, Takeshi Kitano, Miki Nakao, Shun Ichiro Kawabata, Tsuneo Honjo, Yuji Oshima

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)


We successfully purified Trub.TBT-bpα, a tributyltin (TBT) binding protein (bp) of the tiger puffer, Takifugu rubripes. Tiger puffer was injected intraperitoneally with TBT (1.0 mg/kg body weight) and Trub.TBT-bpα was purified from serum by ammonium sulfate fractionation, gel filtration chromatography and polyacrylamide gel electrophoresis. Gel electrophoresis revealed that the Trub.TBT-bpα has a molecular mass of approximately 48.5 kDa and contains at least 40% N-glycan. The deduced 212 amino acid sequence of the protein showed the highest identity (41%, 212 amino acid overlap and E-value: 9e-42) with TBT-binding protein type 1 (TBT-bp1) of Paralichthys olivaceus (Japanese flounder). Analysis of the gene structure of Trub.TBT-bpα suggests that this protein belongs to the lipocalin superfamily, which may be important in the accumulation and elimination of TBT. Phylogenetic analysis suggests that functionalization of TBT-bps has occurred during evolution, and that the functions of this group of proteins might be important for fish survival.

ジャーナルComparative Biochemistry and Physiology - C Toxicology and Pharmacology
出版ステータス出版済み - 1月 2011

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 生理学
  • 水圏科学
  • 動物科学および動物学
  • 毒物学
  • 細胞生物学
  • 健康、毒物学および変異誘発


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