Purification and Characterization of Two Chitinases from the Leaves of Pokeweed(Phytolacca americana)

Moriya Ohta, Takeshi Yamagami, Gunki Funatsu

研究成果: Contribution to journalArticle査読

25 被引用数 (Scopus)

抄録

Two chitinases, designated PLC-A and PLC-B, were purified from the leaves of pokeweed (Phytolacca americana) using DEAE-cellulose column chromatography followed by gel filtration on Sephadex G-75, hydrophobic column chromatography, and ion-exchange FPLC. PLC-A and PLC-B are acidic and basic proteins having molecular masses of 25 and 29kDa, and isoelectric points of 3.7 and 9.5, respectively. On the basis of their partial amino acid sequences, it was seen that PLC-A and PLC-B belong to class II and class III chitinases, respectively. The optimal pH of PLC-A toward glycolchitin is pH 4.5 and hydrolyzed (GlcNAc)4 into 2(GlcNAc)2, and (GlcNAc)5-6 into (GlcNAc)2 and (GlcNAc)3. On the other hand, PLC-B has two optimal pHs at 3 and 7 toward glycolchitin and hydrolyzed (GlcNAc)5 into GlcNAc and (GlcNAc)4, and (GlcNAc)6 into GlcNAc, (GlcNAc)2, and (GlcNAc)4.

本文言語英語
ページ(範囲)656-661
ページ数6
ジャーナルBioscience, biotechnology, and biochemistry
59
4
DOI
出版ステータス出版済み - 1995

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

フィンガープリント

「Purification and Characterization of Two Chitinases from the Leaves of Pokeweed(Phytolacca americana)」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル