Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum

Soha G.R. Abdel-Salam, Masakazu Tsujikura, Masakazu Kondo, Tomonori Somamoto, Miki Nakao

研究成果: ジャーナルへの寄稿記事

抄録

Zymosan, a yeast cell wall preparation that binds activated forms of complement C3, is a useful model target to activate the complement system. In our trial to analyze C3 diversity in Nile tilapia at the protein level using zymosan, we found that a novel 240-kDa serum protein (ZBP-240) also bound to zymosan in addition to C3-derived fragments. In the present study, we aimed to characterize tilapia C3 and ZBP-240, focusing on their immune-related functions. Four distinct C3 isoforms were purified from tilapia serum and shown to possess an intrachain thioester bond. ZBP-240 was also isolated from tilapia serum and examined for its binding properties to various microbial targets. As a result, ZBP-240 showed a wide spectrum of binding to Gram-positive and Gram-negative bacteria and yeasts. Amino acid sequence analysis of CNBr fragments of ZBP-240 suggested that this is a novel protein with no homologous sequence in protein databases. It was also suggested that the binding of ZBP-240 to microbes largely depends on hydrophobic interactions in a divalent-cation-independent manner, and that there may be a divalent-cation-dependent factor that enhances the binding of ZBP-240 in tilapia serum. Interestingly, ZBP-240 showed opsonic activity for tilapia kidney phagocytes at a level comparable to that of C3, implying that ZBP-240 is a novel teleost opsonic serum protein.

元の言語英語
ページ(範囲)301-310
ページ数10
ジャーナルFisheries science
80
発行部数2
DOI
出版物ステータス出版済み - 1 1 2014

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Tilapia (Cichlidae)
zymosan
purification
binding proteins
serum
complement
protein
blood proteins
yeast
cations
cation
yeasts
proteins
binding properties
hydrophobic bonding
phagocytes
Oreochromis niloticus
teleost
sequence homology
Gram-negative bacteria

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum. / Abdel-Salam, Soha G.R.; Tsujikura, Masakazu; Kondo, Masakazu; Somamoto, Tomonori; Nakao, Miki.

:: Fisheries science, 巻 80, 番号 2, 01.01.2014, p. 301-310.

研究成果: ジャーナルへの寄稿記事

Abdel-Salam, Soha G.R. ; Tsujikura, Masakazu ; Kondo, Masakazu ; Somamoto, Tomonori ; Nakao, Miki. / Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum. :: Fisheries science. 2014 ; 巻 80, 番号 2. pp. 301-310.
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