Purification and properties of a low-molecular-weight α-mannosidase from Cellulomonas sp.

Kaoru Takegawa, Satoshi Miki, Takayuki Jikibara, Shojiro Iwahara

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Cellulomonas sp. isolated from soil produces a high level of α-mannosidase (α-mannanase) inductively in culture fluid. The enzyme had two different molecular weight forms, and the properties of the high-molecular-weight form were reported previously (Takegawa, K. et al.: Biochim. Biophys. Acta, 991, 431-437, 1989). The low-molecular-weight α-mannosidase was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was over 150,000 by gel filtration. Unlike the high-molecular-weight form, the low-molecular-weight enzyme readily hydrolyzed α-1,2- and α-1,3-linked mannose chains.

本文言語英語
ページ(範囲)129-131
ページ数3
ジャーナルJournal of Fermentation and Bioengineering
69
2
DOI
出版ステータス出版済み - 1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • バイオエンジニアリング

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