Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate form Candida rugosa

Kenji Sakai, Tatsurokuro Tochikura, Koji Takano, Takashi Tachiki

研究成果: ジャーナルへの寄稿記事

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A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220, 000) consisted of four identical subunits with molecular weight of 58, 000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

元の言語英語
ページ(範囲)2783-2789
ページ数7
ジャーナルAgricultural and Biological Chemistry
52
発行部数11
DOI
出版物ステータス出版済み - 1 1 1988
外部発表Yes

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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