Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate form Candida rugosa

Kenji Sakai, Tatsurokuro Tochikura, Koji Takano, Takashi Tachiki

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220, 000) consisted of four identical subunits with molecular weight of 58, 000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

本文言語英語
ページ(範囲)2783-2789
ページ数7
ジャーナルAgricultural and Biological Chemistry
52
11
DOI
出版ステータス出版済み - 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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