Purification and some properties of a novel ethanol dehydrogenase with high activity against dulcitol 1-phosphate from Salmonella typhimurium IFO 12529

Takahisa Miyamoto, Keishi Yonemura, Makoto Yoshimoto, Shoji Hatano

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

A novel ethanol dehydrogenase with high activity against dulcitol 1-phosphate (D1P-EDH) was purified from Salmonella typhimurium IFO 12529 grown in a medium containing dulcitol as a carbon source. D1P-EDH was purified from a crude extract of S. typhimurium cells by (NH4)2SO4precipitation and column chromatographies on Blue-Cellulofine, Sephacryl S-300, and Zorbax GF-250. D1P-EDH was purified 277-fold with an activity yield of 21.3%. The purified preparation gave a single band on an electrophoregram. The activity staining of the electrophoregram of the (NH4)2SO4precipitate indicated that there was no isozyme of D1P-EDH in the extract. The molecular weight of D1P-EDH was estimated to be 158, 000 by gel filtration and 40, 000 by SDS-polyacrylamide gel electrophoresis. D1P-EDH showed its maximal activity in a pH range from 9.0 to 9.5. D1P-EDH was stable in a pH range from 6.0 to 10.0 and was also stable at 30°C for 120 min. The purified preparation oxidized fructose 6-phosphate and galactose 6-phosphate to the same extent as D1P and oxidized much more ethanol than D1P. D1P-EDH activity was strongly inhibited by p-chloromercuribenzoic acid and NaN3though it was activated by Al3 +, Ba2 +, Ca2 +, and Fe2 +.

本文言語英語
ページ(範囲)3045-3051
ページ数7
ジャーナルAgricultural and Biological Chemistry
55
12
DOI
出版ステータス出版済み - 12 1991

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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