Purification and some properties of an antibacterial factor derived from bacillus subtilis fhc 4o2

Takahisa Miyamoto, Makoto Yoshimoto, Masakazu Tsutsumi, Koji Yamada, Shoji Hatano

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

Bacillus subtilis FHC 402-derived antibacterial factor (abbreviated to BAF), which showed antibacterial activity by its combined use with hexametaphosphate, was purified from a culture broth by means of column chromatography on octyl-Sepharose CL-4B, DEAE-Cellulofine AL and Bio-Gel P-6. BAF was purified 63-fold with an activity yield of 17%. The purified preparation gave a single band on the electrophoregram. BAF may be a glycopeptide containing 22 molecules of 8 different amino acids, 3 hexoses, 1 hexosamine, 1 deoxyribose, and unknown substances. The N-terminal amino acid of the BAF preparation was determined to be tyrosine and the C-terminal amino acid to be proline. Gel filtration estimated the molecular weight of the BAF preparation at 3, 200. The purified BAF preparation was stable in a pH range from 5.0 to 11.0, was also stable on heating, but was unstable at pH 3.0.

本文言語英語
ページ(範囲)1169-1176
ページ数8
ジャーナルAgricultural and Biological Chemistry
50
5
DOI
出版ステータス出版済み - 5月 1986

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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