Purification of a fourth glucosyltransferase from Streptococcus sobrinus

Y. Yamashita, N. Hanada, T. Takehara

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Recently, we found a novel primer-independent, water-soluble glucan synthase as a fourth glucosyltransferase (GTF) in a culture supernatant of strain AHT-k of Streptococcus sobrinus (Y. Yamashita, N. Hanada, and T. Takehara, Biochem. Biophys. Res. Commun. 150:687-693, 1988). In the present study, four kinds of purified GTFs, including the novel GTF, were prepared. They were composed of two-primer-dependent GTFs and two primer-independent GTFs. Of the primer-dependent GTFs, one was a water-insoluble glucan synthase and the other was a water-soluble glucan synthase; both of the primer-independent GTFs were water-soluble glucan synthases (GTF-Sis). Using antisera against four purified GTFs, we concluded that the immunological properties of each were completely different from those of the others. Additionally, it was shown that the novel GTF-Si, which was previously shown to have a molecular weight of 137,000, was proteolytically degraded and could be isolated at a molecular weight of 152,000 and that Streptococcus cricetus secreted an enzyme that immunologically cross-reacted with GTF-Si. While the product of the novel GTF-Si was not an effective primer for both of the primer-dependent enzymes (water-soluble and -insoluble glucan synthases), the product of the enzyme affected the molecular size of the products of the other GTF-Sis.

元の言語英語
ページ(範囲)6265-6270
ページ数6
ジャーナルJournal of bacteriology
171
発行部数11
DOI
出版物ステータス出版済み - 1 1 1989

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Streptococcus sobrinus
Glucosyltransferases
Water
Enzymes
Molecular Weight
Streptococcus
Cricetinae
Immune Sera
glucan synthase

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

Purification of a fourth glucosyltransferase from Streptococcus sobrinus. / Yamashita, Y.; Hanada, N.; Takehara, T.

:: Journal of bacteriology, 巻 171, 番号 11, 01.01.1989, p. 6265-6270.

研究成果: ジャーナルへの寄稿記事

Yamashita, Y. ; Hanada, N. ; Takehara, T. / Purification of a fourth glucosyltransferase from Streptococcus sobrinus. :: Journal of bacteriology. 1989 ; 巻 171, 番号 11. pp. 6265-6270.
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