Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

Chisaka Osamu, Araki Kimi, Ochiya Takahiro, Tsurimoto Toshiki, Hiranyawasitte Attatippaholkun Wacharee, Yanaihara Noboru, Matsubara Kenichi

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

本文言語英語
ページ(範囲)183-189
ページ数7
ジャーナルGene
60
2-3
DOI
出版ステータス出版済み - 1987

All Science Journal Classification (ASJC) codes

  • 遺伝学

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