Quality control of traditional chinese medicine by monoclonal antibody method

Ming Ying Shang, Min Tian, Hiroyuki Tanaka, Xiao Wei Li, Shao Qing Cai, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

In a previous study, we reported the preparation, characterization, variation, specificity, and sensitivity of an anti-aristolochic acid-II (AA-II) monoclonal antibody. The preparation procedure was as follows. AA-II conjugated with bovine serum albumin was used as an antigen for immunizing BALB/c mice. Splenocytes isolated from the immunized mice were fused with an aminopterin-sensitive mouse myeloma cell line to produce hybridoma cells secreting a monoclonal antibody (MAb) against AA-II. The selected MAb was subsequently cloned. Hapten number, isotype, and an estimated dissociation constant (KD) of the secreted MAb were determined. This MAb was used to establish an ELISA method. The linear range was 0.19-13 μg/ml. Anti-AA-II MAb showed extremely high specificity for AA-II, low crossreactivity (CR) against other AAs or aristololactam-I, and negligible CR (<0.5%) toward other natural compounds with different chemical structures. This study describes the successful application of the ELISA method using anti-AA-II MAb to determine AA-II concentration in several crude drugs derived from Aristolochia species. The highest AA-II concentration (2.82 μg/mg) was observed in the stem of A. manshuriensis, followed by that in the fruit of A. contorta (0.81 μg/mg). In case of A. indica, AA-II concentration in the root was higher than that in the aerial parts. These data indicated that the established ELISA method can be used for the quality control of crude drugs derived from Aristolochia plants.

元の言語英語
ページ(範囲)60-65
ページ数6
ジャーナルCurrent Drug Discovery Technologies
8
発行部数1
DOI
出版物ステータス出版済み - 3 1 2011

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Chinese Traditional Medicine
Quality Control
Monoclonal Antibodies
Aristolochia
Enzyme-Linked Immunosorbent Assay
Aminopterin
aristolochic acid II
Haptens
Hybridomas
Bovine Serum Albumin
Pharmaceutical Preparations
Fruit
Antigens
Sensitivity and Specificity
Cell Line

All Science Journal Classification (ASJC) codes

  • Drug Discovery

これを引用

Quality control of traditional chinese medicine by monoclonal antibody method. / Shang, Ming Ying; Tian, Min; Tanaka, Hiroyuki; Li, Xiao Wei; Cai, Shao Qing; Shoyama, Yukihiro.

:: Current Drug Discovery Technologies, 巻 8, 番号 1, 01.03.2011, p. 60-65.

研究成果: ジャーナルへの寄稿記事

Shang, Ming Ying ; Tian, Min ; Tanaka, Hiroyuki ; Li, Xiao Wei ; Cai, Shao Qing ; Shoyama, Yukihiro. / Quality control of traditional chinese medicine by monoclonal antibody method. :: Current Drug Discovery Technologies. 2011 ; 巻 8, 番号 1. pp. 60-65.
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