Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds

Saori Shiota, Shunsuke Yamamoto, Ayane Shimomura, Akio Ojida, Takashi Nishino, Tatsuo Maruyama

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

We quantified amino groups displayed on inorganic and organic surfaces in aqueous solution using different types of cleavable fluorescent compounds and an aldehyde dye. The cleavable fluorescent compounds were designed to bind covalently to amino groups and then liberated under specific conditions. Among the investigated materials, cleavable coumarin was most appropriate for the quantification of amino groups on silica and resin surfaces. The developed method can measure small amounts (∼pmol/cm2) of amino groups on a flat polymeric surface, detecting only amino groups that are exposed to aqueous solution and available for surface immobilization of ligands and biomolecules.

元の言語英語
ページ(範囲)8824-8829
ページ数6
ジャーナルLangmuir
31
発行部数32
DOI
出版物ステータス出版済み - 7 29 2015

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solid surfaces
aqueous solutions
Biomolecules
Aldehydes
Silicon Dioxide
immobilization
Coloring Agents
aldehydes
Dyes
Resins
resins
Ligands
Silica
dyes
silicon dioxide
ligands

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

これを引用

Shiota, S., Yamamoto, S., Shimomura, A., Ojida, A., Nishino, T., & Maruyama, T. (2015). Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds. Langmuir, 31(32), 8824-8829. https://doi.org/10.1021/acs.langmuir.5b02548

Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds. / Shiota, Saori; Yamamoto, Shunsuke; Shimomura, Ayane; Ojida, Akio; Nishino, Takashi; Maruyama, Tatsuo.

:: Langmuir, 巻 31, 番号 32, 29.07.2015, p. 8824-8829.

研究成果: ジャーナルへの寄稿記事

Shiota, S, Yamamoto, S, Shimomura, A, Ojida, A, Nishino, T & Maruyama, T 2015, 'Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds', Langmuir, 巻. 31, 番号 32, pp. 8824-8829. https://doi.org/10.1021/acs.langmuir.5b02548
Shiota, Saori ; Yamamoto, Shunsuke ; Shimomura, Ayane ; Ojida, Akio ; Nishino, Takashi ; Maruyama, Tatsuo. / Quantification of Amino Groups on Solid Surfaces Using Cleavable Fluorescent Compounds. :: Langmuir. 2015 ; 巻 31, 番号 32. pp. 8824-8829.
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