Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture

Hiroki Ikeda, Ana Godinho-Santos, Sylvie Rato, Bénédicte Vanwalscappel, François Clavel, Kazuyuki Aihara, Shingo Iwami, Fabrizio Mammano

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.

元の言語英語
記事番号11761
ジャーナルScientific reports
5
DOI
出版物ステータス出版済み - 6 29 2015

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Interferons
Antiviral Agents
HIV-1
Cell Culture Techniques
HIV
Viruses
Interferon Type I
Tropism
Growth
Infection
Genes

All Science Journal Classification (ASJC) codes

  • General

これを引用

Ikeda, H., Godinho-Santos, A., Rato, S., Vanwalscappel, B., Clavel, F., Aihara, K., ... Mammano, F. (2015). Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. Scientific reports, 5, [11761]. https://doi.org/10.1038/srep11761

Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. / Ikeda, Hiroki; Godinho-Santos, Ana; Rato, Sylvie; Vanwalscappel, Bénédicte; Clavel, François; Aihara, Kazuyuki; Iwami, Shingo; Mammano, Fabrizio.

:: Scientific reports, 巻 5, 11761, 29.06.2015.

研究成果: ジャーナルへの寄稿記事

Ikeda, H, Godinho-Santos, A, Rato, S, Vanwalscappel, B, Clavel, F, Aihara, K, Iwami, S & Mammano, F 2015, 'Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture', Scientific reports, 巻. 5, 11761. https://doi.org/10.1038/srep11761
Ikeda H, Godinho-Santos A, Rato S, Vanwalscappel B, Clavel F, Aihara K その他. Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. Scientific reports. 2015 6 29;5. 11761. https://doi.org/10.1038/srep11761
Ikeda, Hiroki ; Godinho-Santos, Ana ; Rato, Sylvie ; Vanwalscappel, Bénédicte ; Clavel, François ; Aihara, Kazuyuki ; Iwami, Shingo ; Mammano, Fabrizio. / Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. :: Scientific reports. 2015 ; 巻 5.
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