Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Miyako Udono, Keishi Kadooka, Shuntaro Yamashita, Yoshinori Katakura

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Until now, various stimuli as well as serial passaging have been known to induce cellular senescence in normal human diploid fibroblasts. However, in many cases, we have encountered difficulty in quantitatively analyzing the cellular senescence phenotypes of senescent cells in a physiological condition. High-content screening (HCS)-based image analysis is becoming an important and powerful research tool. In the present study, an automated and quantitative cellular image-analysis system was employed to quantify the cellular senescence phenotypes induced in normal human diploid fibroblasts, TIG-1 cells, and found to be a powerful tool in the cellular senescence study.

元の言語英語
ページ(範囲)383-388
ページ数6
ジャーナルMethods
56
発行部数3
DOI
出版物ステータス出版済み - 3 1 2012

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Cell Aging
Fibroblasts
Image analysis
Phenotype
Imaging techniques
Diploidy
Chemical analysis
Screening
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. / Udono, Miyako; Kadooka, Keishi; Yamashita, Shuntaro; Katakura, Yoshinori.

:: Methods, 巻 56, 番号 3, 01.03.2012, p. 383-388.

研究成果: ジャーナルへの寄稿記事

Udono, Miyako ; Kadooka, Keishi ; Yamashita, Shuntaro ; Katakura, Yoshinori. / Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. :: Methods. 2012 ; 巻 56, 番号 3. pp. 383-388.
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