Quantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in the plasma of wistar rats during the oral glucose tolerance test

Si Jing Chen, Chiwa Aikawa, Toshiro Matsui

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

The purpose of this study was to gain insight into the production behavior of free adducts of advanced glycation end-products (AGEs) in Wistar rats under acute hyperglycemic conditions. Five AGE-free adducts as well as their precursors (i.e., highly reactive carbonyl intermediates of methylglyoxal and glyoxal) in rat plasma were quantitatively determined at greater than nanomolar levels using the liquid chromatography/ tandem mass spectrometry method coupled with 2,4,6-trinitrobenzene sulfonate and 2,3-diaminonaphthalene derivatization techniques. An oral glucose (2 g/kg dose) tolerance test to 10-week-old Wistar rats provided evidence that the plasma levels of diabetes-related metabolites did not change acutely within 120 min, irrespective of increasing blood glucose levels.

本文言語英語
ページ(範囲)336-339
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
38
2
DOI
出版ステータス出版済み - 2 1 2015

All Science Journal Classification (ASJC) codes

  • 薬理学
  • 薬科学

フィンガープリント

「Quantitative analysis of methylglyoxal, glyoxal and free advanced glycation end-products in the plasma of wistar rats during the oral glucose tolerance test」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル