Quantitative assessment of mobile protein levels in human knee synovial fluid: Feasibility of chemical exchange saturation transfer (proteinCEST) MRI of osteoarthritis

Guang Jia, Yukihisa Takayama, David C. Flanigan, Christopher C. Kaeding, Jinyuan Zhou, Ajit Chaudhari, Daniel Clark, Steffen Sammet, Jiachao Liang, Seongjin Choi, Michael V. Knopp

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Purpose: To establish the feasibility of chemical exchange saturation transfer (proteinCEST) MRI in the differentiation of osteoarthritis (OA) knee joints from non-OA joints by detecting mobile protein and peptide levels in synovial fluid by determining their relative distribution. Materials and Methods: A total of 25 knees in 11 men and 12 women with knee injuries were imaged using whole knee joint proteinCEST MRI sequence at 3 T. The joint synovial fluid was segmented and the asymmetric magnetization transfer ratio at 3.5 ppm MTRasym (3.5 ppm) was calculated to assess protein content in the synovial fluid. The 85th percentile of synovial fluid MTRasym (3.5 ppm) distribution profile was compared using the independent Student's t test. The diagnostic performance of the 85th percentile of synovial fluid MTRasym (3.5 ppm) in differentiating OA and non-OA knee joints was evaluated. Results: The 85th percentile of synovial fluid MTRasym (3.5 ppm) in knee joints with OA was 8.6%±3.4% and significantly higher than that in the knee joints without OA (6.3%±1.4%, P<05). A knee joint with an 85th percentile of synovial fluid MTRasym (3.5 ppm) greater than 7.7% was considered to be an OA knee joint. With the threshold, the sensitivity, specificity and overall accuracy for differentiating knee joints with OA from the joints without OA were 54% (7/13), 92% (11/12) and 72% (18/25), respectively. Conclusion: proteinCEST MRI appears feasible as a quantitative methodology to determine mobile protein levels in synovial fluid and identify patterns characteristic for OA disease.

本文言語英語
ページ(範囲)335-341
ページ数7
ジャーナルMagnetic Resonance Imaging
29
3
DOI
出版ステータス出版済み - 4 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生体医工学
  • 放射線学、核医学およびイメージング

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