Quantitative dynamics of chromatin remodeling during germ cell specification from mouse embryonic stem cells

Kazuki Kurimoto, Yukihiro Yabuta, Katsuhiko Hayashi, Hiroshi Ohta, Hiroshi Kiyonari, Tadahiro Mitani, Yoshinobu Moritoki, Kenjiro Kohri, Hiroshi Kimura, Takuya Yamamoto, Yuki Katou, Katsuhiko Shirahige, Mitinori Saitou

研究成果: ジャーナルへの寄稿記事

77 引用 (Scopus)

抄録

Germ cell specification is accompanied by epigenetic remodeling, the scale and specificity of which are unclear. Here, we quantitatively delineate chromatin dynamics during induction of mouse embryonic stem cells (ESCs) to epiblast-like cells (EpiLCs) and from there into primordial germ cell-like cells (PGCLCs), revealing large-scale reorganization of chromatin signatures including H3K27me3 and H3K9me2 patterns. EpiLCs contain abundant bivalent gene promoters characterized by low H3K27me3, indicating a state primed for differentiation. PGCLCs initially lose H3K4me3 from many bivalent genes but subsequently regain this mark with concomitant upregulation of H3K27me3, particularly at developmental regulatory genes. PGCLCs progressively lose H3K9me2, including at lamina-associated perinuclear heterochromatin, resulting in changes in nuclear architecture. T recruits H3K27ac to activate BLIMP1 and early mesodermal programs during PGCLC specification, which is followed by BLIMP1-mediated repression of a broad range of targets, possibly through recruitment and spreading of H3K27me3. These findings provide a foundation for reconstructing regulatory networks of the germline epigenome.

元の言語英語
ページ(範囲)517-532
ページ数16
ジャーナルCell stem cell
16
発行部数5
DOI
出版物ステータス出版済み - 5 7 2015

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Chromatin Assembly and Disassembly
Germ Cells
Germ Layers
Chromatin
Developmental Genes
Heterochromatin
Regulator Genes
Epigenomics
Genes
Mouse Embryonic Stem Cells
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

これを引用

Quantitative dynamics of chromatin remodeling during germ cell specification from mouse embryonic stem cells. / Kurimoto, Kazuki; Yabuta, Yukihiro; Hayashi, Katsuhiko; Ohta, Hiroshi; Kiyonari, Hiroshi; Mitani, Tadahiro; Moritoki, Yoshinobu; Kohri, Kenjiro; Kimura, Hiroshi; Yamamoto, Takuya; Katou, Yuki; Shirahige, Katsuhiko; Saitou, Mitinori.

:: Cell stem cell, 巻 16, 番号 5, 07.05.2015, p. 517-532.

研究成果: ジャーナルへの寄稿記事

Kurimoto, K, Yabuta, Y, Hayashi, K, Ohta, H, Kiyonari, H, Mitani, T, Moritoki, Y, Kohri, K, Kimura, H, Yamamoto, T, Katou, Y, Shirahige, K & Saitou, M 2015, 'Quantitative dynamics of chromatin remodeling during germ cell specification from mouse embryonic stem cells', Cell stem cell, 巻. 16, 番号 5, pp. 517-532. https://doi.org/10.1016/j.stem.2015.03.002
Kurimoto, Kazuki ; Yabuta, Yukihiro ; Hayashi, Katsuhiko ; Ohta, Hiroshi ; Kiyonari, Hiroshi ; Mitani, Tadahiro ; Moritoki, Yoshinobu ; Kohri, Kenjiro ; Kimura, Hiroshi ; Yamamoto, Takuya ; Katou, Yuki ; Shirahige, Katsuhiko ; Saitou, Mitinori. / Quantitative dynamics of chromatin remodeling during germ cell specification from mouse embryonic stem cells. :: Cell stem cell. 2015 ; 巻 16, 番号 5. pp. 517-532.
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