Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method

Junya Suehiro, Ryuichi Yatsunami, Ryo Hamada, Masanori Hara

研究成果: Contribution to journalArticle査読

133 被引用数 (Scopus)

抄録

We describe a new technique that realizes the quantitative estimation of the biological cell concentration in an aqueous medium. The proposed dielectrophoretic impedance measurement method utilizes the positive dielectrophoretic force to capture suspended biological particles onto an interdigitated micro-electrode array in pearl-chain formation. Higher cell concentration results in faster development of the pearl chains, which are electrically connected in parallel within the electrode gap to increase the conductance and capacitance between the electrodes. By monitoring temporal variation of the electrical impedance, it is possible to quantitatively evaluate the cell population according to a theoretical model of the cell collection process. It has been demonstrated that a suspension liquid of Escherichia coli could be accurately assayed in about 10 min at 105 cm-3 concentration.

本文言語英語
ページ(範囲)2814-2820
ページ数7
ジャーナルJournal of Physics D: Applied Physics
32
21
DOI
出版ステータス出版済み - 11 7 1999

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 音響学および超音波学
  • 表面、皮膜および薄膜

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