Quantitative single-nucleotide polymorphism analysis in secondary-structured DNA by affinity capillary electrophoresis using a polyethylene glycol-peptide nucleic acid block copolymer

Harumi Tsukada, Lal Mohan Kundu, Yukiharu Matsuoka, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

To estimate allele frequency of single-nucleotide polymorphisms (SNPs) in pooled DNAs with secondary structures, an affinity capillary electrophoresis was developed using an allele-specific peptide nucleic acid probe modified with polyethylene glycol. This probe disrupted secondary structures of DNA analytes and hybridized to them during electrophoresis. Such DNA-binding capability allowed separation of the folded analytes with a single-base difference within 20 min. The feed ratio of the target allele was evaluated by calculating the peak area ratio. The averaged difference between the feed and observed ratio was 1.5%. This method should be of general applicability to quantitative SNP analyses.

元の言語英語
ページ(範囲)150-152
ページ数3
ジャーナルAnalytical Biochemistry
433
発行部数2
DOI
出版物ステータス出版済み - 2 15 2013

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Peptide Nucleic Acids
Capillary electrophoresis
Capillary Electrophoresis
Polymorphism
Block copolymers
Single Nucleotide Polymorphism
Nucleotides
DNA
Alleles
Nucleic Acid Probes
Electrophoresis
Gene Frequency

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

これを引用

Quantitative single-nucleotide polymorphism analysis in secondary-structured DNA by affinity capillary electrophoresis using a polyethylene glycol-peptide nucleic acid block copolymer. / Tsukada, Harumi; Kundu, Lal Mohan; Matsuoka, Yukiharu; Kanayama, Naoki; Takarada, Tohru; Maeda, Mizuo.

:: Analytical Biochemistry, 巻 433, 番号 2, 15.02.2013, p. 150-152.

研究成果: ジャーナルへの寄稿記事

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AU - Takarada, Tohru

AU - Maeda, Mizuo

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