Quantum dots for labeling adipose tissue-derived stem cells

Hiroshi Yukawa, Shogo Mizufune, Chiharu Mamori, Yukimasa Kagami, Koichi Oishi, Noritada Kaji, Yukihiro Okamoto, Manabu Takeshi, Hirofumi Noguchi, Yoshinobu Baba, Michinari Hamaguchi, Nobuyuki Hamajima, Shuji Hayashi

研究成果: Contribution to journalArticle査読

42 被引用数 (Scopus)

抄録

Adipose tissue-derived stem cells (ASCs) have a self-renewing ability and can be induced to differentiate into various types of mesenchymal tissue. Because of their potential for clinical application, it has become desirable to label the cells for tracing transplanted cells and for in vivo imaging. Quantum dots (QDs) are novel inorganic probes that consist of CdSe/ZnS-core/shell semiconductor nanocrystals and have recently been explored as fluorescent probes for stem cell labeling. In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and osteogenic cells with red fluorescence after transfection with QDs655, thus suggesting that the cells retain their potential for differentiation even after transfected with QDs655. These data suggest that QDs could be utilized for the labeling of ASCs.

本文言語英語
ページ(範囲)591-599
ページ数9
ジャーナルCell Transplantation
18
5-6
DOI
出版ステータス出版済み - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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