Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity

Yohei Sasagawa, Itoshi Nikaido, Tetsutaro Hayashi, Hiroki Danno, Kenichiro D. Uno, Takeshi Imai, Hiroki R. Ueda

研究成果: ジャーナルへの寄稿記事

172 引用 (Scopus)

抄録

Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.

元の言語英語
記事番号R31
ジャーナルGenome biology
14
発行部数4
DOI
出版物ステータス出版済み - 4 17 2013
外部発表Yes

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RNA Sequence Analysis
Quartz
quartz
gene expression
RNA
sequence analysis
Gene Expression
cells
methodology
cell cycle
Cell Cycle
method
reproducibility

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

これを引用

Quartz-Seq : A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity. / Sasagawa, Yohei; Nikaido, Itoshi; Hayashi, Tetsutaro; Danno, Hiroki; Uno, Kenichiro D.; Imai, Takeshi; Ueda, Hiroki R.

:: Genome biology, 巻 14, 番号 4, R31, 17.04.2013.

研究成果: ジャーナルへの寄稿記事

Sasagawa, Yohei ; Nikaido, Itoshi ; Hayashi, Tetsutaro ; Danno, Hiroki ; Uno, Kenichiro D. ; Imai, Takeshi ; Ueda, Hiroki R. / Quartz-Seq : A highly reproducible and sensitive single-cell RNA sequencing method, reveals nongenetic gene-expression heterogeneity. :: Genome biology. 2013 ; 巻 14, 番号 4.
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