Quick quantitative analysis of gene dosages associated with prognosis in neuroblastoma

Tatsuro Tajiri, Shinji Tanaka, Kumiko Shono, Yoshiaki Kinoshita, Yoshimitu Fujii, Sachiyo Suita, Kenji Ihara, Toshiro Hara

研究成果: Contribution to journalArticle査読

43 被引用数 (Scopus)

抄録

The amplification of the N-myc gene and a gain of the chromosome 17q arm correlate with an unfavorable outcome in patients with neuroblastoma. In this study, we determined the gene dosage of the N-myc gene (located at 2p24) and Survivin gene (located at 17q25) using the p53 gene (located at 17p13) as the internal control gene by the TaqMan polymerase chain reaction (PCR)-based gene dosage analysis in 25 neuroblastoma samples. Based on the assumption that the gene dosages of each gene of a normal individual lymphocytes are 1.0, 11 of the 25 cases with a corrected gene dosage of N-myc (N-myc/p53) of more than 2.0 had a more unfavorable prognosis than the 14 cases with a N-myc gene dosage of less than 2.0 (5-year survival rate: 18 vs. 71%, P < 0.01). Ten of 25 cases with a corrected Survivin gene dosage (Survivin/p53) of more than 2.0 had a more unfavorable prognosis than the 15 cases with a Survivin gene dosage of less than 2.0 (5-year survival rate: 10 vs. 67%, P < 0.01). This quantitative PCR system is considered to be useful for quickly and accurately evaluating the degree of malignancy of neuroblastoma in order to select the optimal treatment.

本文言語英語
ページ(範囲)89-94
ページ数6
ジャーナルCancer Letters
166
1
DOI
出版ステータス出版済み - 5 10 2001

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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