RAD5a ubiquitin ligase is involved in ubiquitination of Arabidopsis thaliana proliferating cell nuclear antigen

Wojciech Strzalka, Filip Bartnicki, Katarzyna Pels, Agata Jakubowska, Toshiki Tsurimoto, Katsunori Tanaka

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

The proliferating cell nuclear antigen (PCNA) is post-translationally modified by ubiquitin in yeast and mammalian cells. It is widely accepted that in yeast mono- and polyubiquitinated PCNA is involved in distinct pathways of DNA postreplication repair. This study showed an interaction between plant ubiquitin and PCNA in the plant cell. Using different approaches, it was demonstrated that Arabidopsis RAD5a ubiquitin ligase is involved in the post-translational modification of plant PCNA. A detailed analysis of the properties of selected Arabidopsis ubiquitin-conjugating enzymes (AtUBC) has shown that a plant homologue of yeast RAD6 (AtUBC2) is sufficient to monoubiquitinate AtPCNA in the absence of ubiquitin ligase. Using different combinations of selected AtUBC proteins together with AtRAD5a, it was demonstrated that plants have potential to use different pathways to ubiquitinate PCNA. The analysis of Arabidopsis PCNA1 and PCNA2 did not demonstrate substantial differences in the ubiquitination pattern between these two proteins. The major ubiquitination target of Arabidopsis PCNA, conserved in eukaryotes, is lysine 164. Taken together, the presented results clearly demonstrate the involvement of Arabidopsis UBC and RAD5a proteins in the ubiquitination of plant PCNA at lysine 164. The data show the complexity of the plant ubiquitination system and open new questions about its regulation in the plant cell.

元の言語英語
ページ(範囲)859-869
ページ数11
ジャーナルJournal of Experimental Botany
64
発行部数4
DOI
出版物ステータス出版済み - 3 1 2013

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proliferating cell nuclear antigen
Ubiquitination
Proliferating Cell Nuclear Antigen
Ligases
ubiquitin
Ubiquitin
ligases
Arabidopsis
Arabidopsis thaliana
Plant Cells
Yeasts
Lysine
yeasts
lysine
Ubiquitin-Conjugating Enzymes
ubiquitin-protein ligase
Post Translational Protein Processing
proteins
ubiquitination
Eukaryota

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

RAD5a ubiquitin ligase is involved in ubiquitination of Arabidopsis thaliana proliferating cell nuclear antigen. / Strzalka, Wojciech; Bartnicki, Filip; Pels, Katarzyna; Jakubowska, Agata; Tsurimoto, Toshiki; Tanaka, Katsunori.

:: Journal of Experimental Botany, 巻 64, 番号 4, 01.03.2013, p. 859-869.

研究成果: ジャーナルへの寄稿記事

Strzalka, Wojciech ; Bartnicki, Filip ; Pels, Katarzyna ; Jakubowska, Agata ; Tsurimoto, Toshiki ; Tanaka, Katsunori. / RAD5a ubiquitin ligase is involved in ubiquitination of Arabidopsis thaliana proliferating cell nuclear antigen. :: Journal of Experimental Botany. 2013 ; 巻 64, 番号 4. pp. 859-869.
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