Radiation-induced centrosome overduplication and multiple mitotic spindles in human tumor cells

Norihiro Sato, Kazuhiro Mizumoto, Masafumi Nakamura, Masao Tanaka

研究成果: Contribution to journalArticle査読

74 被引用数 (Scopus)


The centrosome is a highly regulated organelle and its proper duplication is indispensable for the formation of bipolar mitotic spindles and balanced chromosome segregation. To elucidate a possible linkage between centrosome duplication and radiation-induced nuclear damage, we examined centrosome dynamics in U2-OS osteosarcoma cells following γ-irradiation. Nearly all control cells contained one or two centrosomes, and at mitosis more than 97% of the cells displayed typical bipolar spindles. In contrast, over 20% of cells at 48 h after 10 Gy γ-irradiation contained more than two centrosomes, and 60% of the mitotic cells showed aberrant spindles organized by multiple poles. Remarkably, the cells with multiple centrosomes frequently exhibited changes in size and/or morphology of the nucleus, including micronuclei formation. We conclude that abnormal centrosome duplication could be one of the key events involved in nuclear fragmentation and perhaps even cell death following irradiation. (C) 2000 Academic Press.

ジャーナルExperimental Cell Research
出版ステータス出版済み - 3 15 2000

All Science Journal Classification (ASJC) codes

  • Cell Biology

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