Radiation-inducible TNF-α gene expression under stress-inducible promoter gadd 153 for cancer therapy

Akira Ito, Masashige Shinkai, Kazumi Hakamada, Hiroyuki Honda, Takeshi Kobayashi

研究成果: Contribution to journalArticle

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We demonstrated the effectiveness of radiation-inducible expression of the TNF-α gene for cancer therapy in vitro. The TNF-α gene under the control of the stress-inducible promoter, gadd 153, was introduced into the human glioma cell line, U251-SP. Without cobalt-60 gamma irradiation, no cytotoxicity against the transfected cells was observed. When the transfected cells were irradiated with 10 or 20 gray (Gy), the gadd 153 promoter was highly induced and the expression level of TNF-α increased. Five days after the irradiation, the TNF-α productions of each cell irradiated with 10 and 20 Gy were 30 and 100 times higher than the basal level, respectively. The cytotoxicities against the transfected cells 5 d after irradiation with 10 and 20 Gy were 79% or 91%, respectively, which are much higher than those against the nontransfected cells that were irradiated at the same dose (43% and 78%, respectively). These results demonstrate that the gadd 153-TNF-α system may be an effective tool for radiosurgery of malignant brain tumors.

元の言語英語
ページ(範囲)598-601
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
92
発行部数6
DOI
出版物ステータス出版済み - 1 1 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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