RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles

Kyosuke Isoda, Naoki Kanayama, Daisuke Miyamoto, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

32 引用 (Scopus)

抄録

A diblock copolymer containing a single-stranded DNA and a temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm-b-DNA) was synthesized. The PNIPAAm segment was obtained with a low polydispersity by reversible addition-fragmentation chain transfer polymerization. The number-averaged molecular weight and the molecular weight distribution of the PNIPAAm were determined with size exclusion chromatography. PNIPAAm-b-DNA was prepared through the Michael addition reaction of thiol-terminated PNIPAAm to 5′-maleimide-modified DNA. The lower critical solution temperature (LCST) of the copolymer was determined with differential scanning calorimetry. PNIPAAm-b-DNA self-assembled above the LCST into the formation of a polymer micelle with a hydrophobic PNIPAAm core surrounded by a hydrophilic DNA corona. Both light scattering and transmittance measurements revealed that the colloidal stability of the single-stranded DNA-carrying polymer micelles was enormously decreased when the complementary DNA was added into the micelle's dispersion to form the fully matched DNA duplex on the surface. It was also found that the colloidal stability of the micelle was extremely enhanced when the 5′-terminal-mismatched DNA was added to form a single-base mismatch at the interface between the DNA corona and the dispersal medium. The higher flexibility of the DNA terminus in the corona presumably increased the entropic repulsion between the micelles.

元の言語英語
ページ(範囲)367-371
ページ数5
ジャーナルReactive and Functional Polymers
71
発行部数3
DOI
出版物ステータス出版済み - 3 1 2011
外部発表Yes

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Micelles
Block copolymers
Polymers
DNA
polymer
Temperature
temperature
Single-Stranded DNA
corona
Molecular Weight
poly-N-isopropylacrylamide
Addition reactions
Size exclusion chromatography
Differential Scanning Calorimetry
Polydispersity
Molecular weight distribution
Sulfhydryl Compounds
Polymerization
Light scattering
Gel Chromatography

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

これを引用

RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles. / Isoda, Kyosuke; Kanayama, Naoki; Miyamoto, Daisuke; Takarada, Tohru; Maeda, Mizuo.

:: Reactive and Functional Polymers, 巻 71, 番号 3, 01.03.2011, p. 367-371.

研究成果: ジャーナルへの寄稿記事

Isoda, Kyosuke ; Kanayama, Naoki ; Miyamoto, Daisuke ; Takarada, Tohru ; Maeda, Mizuo. / RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles. :: Reactive and Functional Polymers. 2011 ; 巻 71, 番号 3. pp. 367-371.
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