Raloxifene induces nucleolar translocation of the estrogen receptor

Mika Gushima, Hisaya Kawate, Keizo Ohnaka, Masatoshi Nomura, Ryoichi Takayanagi

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Raloxifene (RLX), a selective estrogen receptor modulator (SERM), binds to the estrogen receptor alpha (ERα) and acts as an agonist in some tissues, and as an antagonist in others. To clarify the molecular mechanism underlying the tissue specificity of SERMs, we examined the intracellular localization of ERα using a green fluorescent protein (GFP)-tagged protein in culture cells from various tissues. Although ERα formed intranuclear foci in the presence of estradiol (E2), RLX translocated ERα into the nucleoli in breast cancer cell lines. This phenomenon was not observed in cells from other tissues. Immunofluorescence staining revealed that endogenous ERα was also translocated into the nucleoli in the presence of RLX. Mutation analyses demonstrate that helix 12 of ERα is essential to the nucleolar translocation of ERα. These results suggest that translocation of ERα into the nucleoli is RLX-specific and is a key event for RLX-induced growth repression of mammary gland cells.

元の言語英語
ページ(範囲)14-22
ページ数9
ジャーナルMolecular and Cellular Endocrinology
319
発行部数1-2
DOI
出版物ステータス出版済み - 5 5 2010

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Estrogen Receptor alpha
Estrogen Receptors
Tissue
Selective Estrogen Receptor Modulators
Organ Specificity
Raloxifene Hydrochloride
Human Mammary Glands
Green Fluorescent Proteins
Cell culture
Fluorescent Antibody Technique
Estradiol
Cell Culture Techniques
Cells
Staining and Labeling
Breast Neoplasms
Cell Line
Mutation
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

これを引用

Raloxifene induces nucleolar translocation of the estrogen receptor. / Gushima, Mika; Kawate, Hisaya; Ohnaka, Keizo; Nomura, Masatoshi; Takayanagi, Ryoichi.

:: Molecular and Cellular Endocrinology, 巻 319, 番号 1-2, 05.05.2010, p. 14-22.

研究成果: ジャーナルへの寄稿記事

Gushima, Mika ; Kawate, Hisaya ; Ohnaka, Keizo ; Nomura, Masatoshi ; Takayanagi, Ryoichi. / Raloxifene induces nucleolar translocation of the estrogen receptor. :: Molecular and Cellular Endocrinology. 2010 ; 巻 319, 番号 1-2. pp. 14-22.
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