@article{ab9a3eee65a9488fbe293289c8c454a0,
title = "Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients",
abstract = "ABL tyrosine kinase inhibitor (TKI), imatinib is used for BCR-ABL + leukemias. We developed an automatic method utilizing guanine-quenching probes (QP) to detect 17 kinds of mutations frequently observed in imatinib-resistance. Results were obtained from 100μL of whole blood within 90min by this method. Detected mutations were almost identical between QP method and direct sequencing. Furthermore, the mutation-biased PCR (MBP) was added to the QP method to increase sensitivity, resulting earlier detection of T315I mutation which was insensitive to any ABL TKIs. Thus, the QP and MBP-QP may become useful methods for the management of ABL TKI-treated patients.",
author = "Ruriko Tanaka and Shinya Kimura and Eishi Ashihara and Mariko Yoshimura and Naoto Takahashi and Hisashi Wakita and Kuniaki Itoh and Kaichi Nishiwaki and Kenshi Suzuki and Rina Nagao and Hisayuki Yao and Yoshihiro Hayashi and Sakiko Satake and Hideyo Hirai and Sawada, {Ken ichi} and Ottmann, {Oliver G.} and Melo, {Junia V.} and Taira Maekawa",
note = "Funding Information: We thank Naoko Hashimoto and Mikiko Katakami for technical support. We would also like to thank Jun Kuroki, Atsushi Kitabayashi, Hisako Iso, Hideyoshi Noji and Hideo Kimura, and all the various clinical centres participating in this study. This work was partly supported by Grant-in-Aids for Scientific Research and for the Japan Society for the Promotion of Science (JSPS) fellows, by the Global COE Program “Centre for Frontier Medicine” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by Management Expenses Grants from the Government to the National Cancer Centre of Japan. ",
year = "2011",
month = dec,
day = "22",
doi = "10.1016/j.canlet.2011.08.009",
language = "English",
volume = "312",
pages = "228--234",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",
}