Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients

Ruriko Tanaka; Shinya Kimura; Eishi Ashihara; Mariko Yoshimura; Naoto Takahashi; Hisashi Wakita; Kuniaki Itoh; Kaichi Nishiwaki; Kenshi Suzuki; Rina Nagao; Hisayuki Yao; Yoshihiro Hayashi; Sakiko Satake; Hideyo Hirai; Ken ichi Sawada; Oliver G. Ottmann; Junia V. Melo; Taira Maekawa

doi:10.1016/j.canlet.2011.08.009

Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients

Ruriko Tanaka, Shinya Kimura, Eishi Ashihara, Mariko Yoshimura, Naoto Takahashi, Hisashi Wakita, Kuniaki Itoh, Kaichi Nishiwaki, Kenshi Suzuki, Rina Nagao, Hisayuki Yao, Yoshihiro Hayashi, Sakiko Satake, Hideyo Hirai, Ken ichi Sawada, Oliver G. Ottmann, Junia V. Melo, Taira Maekawa

研究成果: Contribution to journal › Article › 査読

9 被引用数 (Scopus)

抄録

ABL tyrosine kinase inhibitor (TKI), imatinib is used for BCR-ABL ⁺ leukemias. We developed an automatic method utilizing guanine-quenching probes (QP) to detect 17 kinds of mutations frequently observed in imatinib-resistance. Results were obtained from 100μL of whole blood within 90min by this method. Detected mutations were almost identical between QP method and direct sequencing. Furthermore, the mutation-biased PCR (MBP) was added to the QP method to increase sensitivity, resulting earlier detection of T315I mutation which was insensitive to any ABL TKIs. Thus, the QP and MBP-QP may become useful methods for the management of ABL TKI-treated patients.

本文言語	英語
ページ（範囲）	228-234
ページ数	7
ジャーナル	Cancer Letters
巻	312
号	2
DOI	https://doi.org/10.1016/j.canlet.2011.08.009
出版ステータス	出版済み - 12 22 2011
外部発表	はい

All Science Journal Classification (ASJC) codes

腫瘍学
癌研究

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.canlet.2011.08.009

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引用スタイル

Tanaka, R., Kimura, S., Ashihara, E., Yoshimura, M., Takahashi, N., Wakita, H., Itoh, K., Nishiwaki, K., Suzuki, K., Nagao, R., Yao, H., Hayashi, Y., Satake, S., Hirai, H., Sawada, K. I., Ottmann, O. G., Melo, J. V., & Maekawa, T. (2011). Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients. Cancer Letters, 312(2), 228-234. https://doi.org/10.1016/j.canlet.2011.08.009

Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients. / Tanaka, Ruriko; Kimura, Shinya; Ashihara, Eishi; Yoshimura, Mariko; Takahashi, Naoto; Wakita, Hisashi; Itoh, Kuniaki; Nishiwaki, Kaichi; Suzuki, Kenshi; Nagao, Rina; Yao, Hisayuki; Hayashi, Yoshihiro; Satake, Sakiko; Hirai, Hideyo; Sawada, Ken ichi; Ottmann, Oliver G.; Melo, Junia V.; Maekawa, Taira.

In: Cancer Letters, Vol. 312, No. 2, 22.12.2011, p. 228-234.

研究成果: Contribution to journal › Article › 査読

Tanaka, R, Kimura, S, Ashihara, E, Yoshimura, M, Takahashi, N, Wakita, H, Itoh, K, Nishiwaki, K, Suzuki, K, Nagao, R, Yao, H, Hayashi, Y, Satake, S, Hirai, H, Sawada, KI, Ottmann, OG, Melo, JV & Maekawa, T 2011, 'Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients', Cancer Letters, vol. 312, no. 2, pp. 228-234. https://doi.org/10.1016/j.canlet.2011.08.009

Tanaka, Ruriko ; Kimura, Shinya ; Ashihara, Eishi ; Yoshimura, Mariko ; Takahashi, Naoto ; Wakita, Hisashi ; Itoh, Kuniaki ; Nishiwaki, Kaichi ; Suzuki, Kenshi ; Nagao, Rina ; Yao, Hisayuki ; Hayashi, Yoshihiro ; Satake, Sakiko ; Hirai, Hideyo ; Sawada, Ken ichi ; Ottmann, Oliver G. ; Melo, Junia V. ; Maekawa, Taira. / Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients. In: Cancer Letters. 2011 ; Vol. 312, No. 2. pp. 228-234.

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title = "Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients",

abstract = "ABL tyrosine kinase inhibitor (TKI), imatinib is used for BCR-ABL + leukemias. We developed an automatic method utilizing guanine-quenching probes (QP) to detect 17 kinds of mutations frequently observed in imatinib-resistance. Results were obtained from 100μL of whole blood within 90min by this method. Detected mutations were almost identical between QP method and direct sequencing. Furthermore, the mutation-biased PCR (MBP) was added to the QP method to increase sensitivity, resulting earlier detection of T315I mutation which was insensitive to any ABL TKIs. Thus, the QP and MBP-QP may become useful methods for the management of ABL TKI-treated patients.",

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AU - Takahashi, Naoto

AU - Wakita, Hisashi

AU - Itoh, Kuniaki

AU - Nishiwaki, Kaichi

AU - Suzuki, Kenshi

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AU - Hayashi, Yoshihiro

AU - Satake, Sakiko

AU - Hirai, Hideyo

AU - Sawada, Ken ichi

AU - Ottmann, Oliver G.

AU - Melo, Junia V.

AU - Maekawa, Taira

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Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients

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All Science Journal Classification (ASJC) codes

UN SDG

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