Rapid detection of SAG 926delA mutation using real-time polymerase chain reaction

Shigeo Yoshida, Yoko Yamaji, Ayako Yoshida, Yasuhiro Ikeda, Ken Yamamoto, Tatsuro Ishibashi

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Purpose: Mutation 926delA of the arrestin/S-antigen SAG gene is the main cause of Oguchi disease in the Japanese. The purpose of this study was to develop a rapid diagnostic assay to detect mutations in the SAG gene. Methods: Two sequence-specific primers and fluorophore-labeled probes for exon 11 of the SAG gene were designed, and the region spanning the mutations was amplified by polymerase chain reaction (PCR) using the LightCycler detection system (Roche Diagnostics, Mannheim, Germany). The mutations were then identified by melting curve analyses of the hybrid formed between the PCR product and a specific fluorescent probe. Results: We clearly distinguished each SAG genotype (homozygous and heterozygous 926delA and wild type) by the distinct melting peaks at different temperatures. One thermal cycling required approximately 54 min to process, and the results were 100% in concordance with the genotypes determined by DNA sequencing. Conclusions: We have succeeded in developing a rapid method to detect the most frequent mutation in the SAG gene. This method will help in identifying gene mutations associated with Oguchi disease with a rapid and reliable identification or the exclusion of the frequent mutations in the SAG gene.

本文言語英語
ページ(範囲)1552-1557
ページ数6
ジャーナルMolecular vision
12
出版ステータス出版済み - 12 6 2006

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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