Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

Yoshiaki Shinden, Hiroki Ueo, Taro Tobo, Ayako Gamachi, Mitsuaki Utou, Hisateru Komatsu, Sho Nambara, Tomoko Saito, Masami Ueda, Hidenari Hirata, Shotaro Sakimura, Yuki Takano, Ryutaro Uchi, Junji Kurashige, Sayuri Akiyoshi, Tomohiro Iguchi, Hidetoshi Eguchi, Keishi Sugimachi, Yoko Kubota, Yuichiro KaiKenji Shibuta, Yuko Kijima, Heiji Yoshinaka, Shoji Natsugoe, Masaki Mori, Yoshihiko Maehara, Masayo Sakabe, Mako Kamiya, John W. Kakareka, Thomas J. Pohida, Peter L. Choyke, Hisataka Kobayashi, Hiroaki Ueo, Yasuteru Urano, Koshi Mimori

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

元の言語英語
記事番号27525
ジャーナルScientific reports
6
DOI
出版物ステータス出版済み - 6 9 2016

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Rhodamines
Lymph Nodes
Breast Neoplasms
Neoplasm Metastasis
Sentinel Lymph Node Biopsy
Costs and Cost Analysis
Sensitivity and Specificity
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

これを引用

Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green. / Shinden, Yoshiaki; Ueo, Hiroki; Tobo, Taro; Gamachi, Ayako; Utou, Mitsuaki; Komatsu, Hisateru; Nambara, Sho; Saito, Tomoko; Ueda, Masami; Hirata, Hidenari; Sakimura, Shotaro; Takano, Yuki; Uchi, Ryutaro; Kurashige, Junji; Akiyoshi, Sayuri; Iguchi, Tomohiro; Eguchi, Hidetoshi; Sugimachi, Keishi; Kubota, Yoko; Kai, Yuichiro; Shibuta, Kenji; Kijima, Yuko; Yoshinaka, Heiji; Natsugoe, Shoji; Mori, Masaki; Maehara, Yoshihiko; Sakabe, Masayo; Kamiya, Mako; Kakareka, John W.; Pohida, Thomas J.; Choyke, Peter L.; Kobayashi, Hisataka; Ueo, Hiroaki; Urano, Yasuteru; Mimori, Koshi.

:: Scientific reports, 巻 6, 27525, 09.06.2016.

研究成果: ジャーナルへの寄稿記事

Shinden, Y, Ueo, H, Tobo, T, Gamachi, A, Utou, M, Komatsu, H, Nambara, S, Saito, T, Ueda, M, Hirata, H, Sakimura, S, Takano, Y, Uchi, R, Kurashige, J, Akiyoshi, S, Iguchi, T, Eguchi, H, Sugimachi, K, Kubota, Y, Kai, Y, Shibuta, K, Kijima, Y, Yoshinaka, H, Natsugoe, S, Mori, M, Maehara, Y, Sakabe, M, Kamiya, M, Kakareka, JW, Pohida, TJ, Choyke, PL, Kobayashi, H, Ueo, H, Urano, Y & Mimori, K 2016, 'Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green', Scientific reports, 巻. 6, 27525. https://doi.org/10.1038/srep27525
Shinden, Yoshiaki ; Ueo, Hiroki ; Tobo, Taro ; Gamachi, Ayako ; Utou, Mitsuaki ; Komatsu, Hisateru ; Nambara, Sho ; Saito, Tomoko ; Ueda, Masami ; Hirata, Hidenari ; Sakimura, Shotaro ; Takano, Yuki ; Uchi, Ryutaro ; Kurashige, Junji ; Akiyoshi, Sayuri ; Iguchi, Tomohiro ; Eguchi, Hidetoshi ; Sugimachi, Keishi ; Kubota, Yoko ; Kai, Yuichiro ; Shibuta, Kenji ; Kijima, Yuko ; Yoshinaka, Heiji ; Natsugoe, Shoji ; Mori, Masaki ; Maehara, Yoshihiko ; Sakabe, Masayo ; Kamiya, Mako ; Kakareka, John W. ; Pohida, Thomas J. ; Choyke, Peter L. ; Kobayashi, Hisataka ; Ueo, Hiroaki ; Urano, Yasuteru ; Mimori, Koshi. / Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green. :: Scientific reports. 2016 ; 巻 6.
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abstract = "Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97{\%}), specificity (79{\%}) and negative predictive value (99{\%}) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.",
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AU - Shinden, Yoshiaki

AU - Ueo, Hiroki

AU - Tobo, Taro

AU - Gamachi, Ayako

AU - Utou, Mitsuaki

AU - Komatsu, Hisateru

AU - Nambara, Sho

AU - Saito, Tomoko

AU - Ueda, Masami

AU - Hirata, Hidenari

AU - Sakimura, Shotaro

AU - Takano, Yuki

AU - Uchi, Ryutaro

AU - Kurashige, Junji

AU - Akiyoshi, Sayuri

AU - Iguchi, Tomohiro

AU - Eguchi, Hidetoshi

AU - Sugimachi, Keishi

AU - Kubota, Yoko

AU - Kai, Yuichiro

AU - Shibuta, Kenji

AU - Kijima, Yuko

AU - Yoshinaka, Heiji

AU - Natsugoe, Shoji

AU - Mori, Masaki

AU - Maehara, Yoshihiko

AU - Sakabe, Masayo

AU - Kamiya, Mako

AU - Kakareka, John W.

AU - Pohida, Thomas J.

AU - Choyke, Peter L.

AU - Kobayashi, Hisataka

AU - Ueo, Hiroaki

AU - Urano, Yasuteru

AU - Mimori, Koshi

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N2 - Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

AB - Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.

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