Rapid Extraction-HPLC as a Tool for Presumptive Identification of Burkholderia gladioli, B. glumae and B. plantarii, Causal Agents of Various Rice Diseases

Nobuaki Matsuyama, Yasuko Ueda, Kazuhiro Iiyama, Naruto Furuya, Hiroyuki Ura, Abu Ashraf Khan, Matsumoto Masaru

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Rapid extraction-HPLC was performed to estimate its usefulness for presumptive identification of Burkholderia gladioli, B. glumae and B. plantarii. On HPLC chromatograms of 12 isolates of B. gladioli, a distinct peak appeared at Rt 6.2 min without exception. This peak was absent on the chromatograms for 18 isolates of B. glumae and 5 isolates of B. plantarii tested. Benchmark peak at Rt 3.2min was observed on the chromatogram of B. plantarii. Together with the direct colony TLC method, this simplified HPLC analysis will be useful for identification of phytopathogenic bacteria.

本文言語英語
ページ(範囲)265-272
ページ数8
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
42
3-4
出版ステータス出版済み - 3 1 1998

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学

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