TY - JOUR
T1 - Rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis
AU - Chen, Jin
AU - Ni, Yi
AU - Liu, Chenchen
AU - Yamaguchi, Yoshinori
AU - Chen, Qinmiao
AU - Sekine, Shinichi
AU - Zhu, Xifang
AU - Dou, Xiaoming
N1 - Funding Information:
The project was financially supported by the National Natural Science Foundation of China (No. 21305089 ), Grants-in-Aid for Scientific Research of Japan ( A15H038270 [Y.Y.]), and the Fund of East China University of Science and Technology (No. YK0142119 ).
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/μl, respectively.
AB - High-speed capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-end CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/μl, respectively.
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U2 - 10.1016/j.talanta.2016.07.049
DO - 10.1016/j.talanta.2016.07.049
M3 - Article
C2 - 27591633
AN - SCOPUS:84980340196
SN - 0039-9140
VL - 160
SP - 425
EP - 430
JO - Talanta
JF - Talanta
ER -