Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement

Michihiko Nakano, Zhenhao Ding, Hiromichi Kasahara, Junya Suehiro

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.

元の言語英語
記事番号28003
ジャーナルEPL
108
発行部数2
DOI
出版物ステータス出版済み - 10 1 2014

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impedance measurement
deoxyribonucleic acid
polymerase chain reaction
viruses
infectious diseases
marking

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

これを引用

Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement. / Nakano, Michihiko; Ding, Zhenhao; Kasahara, Hiromichi; Suehiro, Junya.

:: EPL, 巻 108, 番号 2, 28003, 01.10.2014.

研究成果: ジャーナルへの寄稿記事

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