Rapid Naked-Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non-Crosslinking Aggregation of DNA-Functionalized Gold Nanoparticles

Yoshitsugu Akiyama, Guoqing Wang, Shota Shiraishi, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Involvement of single-nucleotide polymorphism (SNP) genotyping in healthcare should allow for more effective use of pharmacogenomics. However, user-friendly assays without the requirement of a special instrument still remain unavailable. This study describes naked-eye SNP discrimination in exon 5 of the human cytochrome P450 2C19 monooxygenase gene, CYP2C19*1 (the wild-type allele) and CYP2C19*2 (the variant allele with G681A point mutation). The present assay is composed of allele-specific single-base primer extension and salt-induced aggregation of DNA-modified gold nanoparticles (DNA–AuNPs). Genetic samples extracted from human hair roots are subjected to this assay. The results are verified by direct sequencing. This study should promise the prospective use of DNA–AuNPs in gene diagnosis.

元の言語英語
ページ(範囲)508-512
ページ数5
ジャーナルChemistryOpen
5
発行部数6
DOI
出版物ステータス出版済み - 12 1 2016

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Polymorphism
Gold
Cytochrome P-450 Enzyme System
Assays
Agglomeration
Nanoparticles
DNA
Nucleotides
Genes
Mixed Function Oxygenases
Exons
Salts
Cytochrome P-450 CYP2C19

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

Rapid Naked-Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non-Crosslinking Aggregation of DNA-Functionalized Gold Nanoparticles. / Akiyama, Yoshitsugu; Wang, Guoqing; Shiraishi, Shota; Kanayama, Naoki; Takarada, Tohru; Maeda, Mizuo.

:: ChemistryOpen, 巻 5, 番号 6, 01.12.2016, p. 508-512.

研究成果: ジャーナルへの寄稿記事

Akiyama, Yoshitsugu ; Wang, Guoqing ; Shiraishi, Shota ; Kanayama, Naoki ; Takarada, Tohru ; Maeda, Mizuo. / Rapid Naked-Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non-Crosslinking Aggregation of DNA-Functionalized Gold Nanoparticles. :: ChemistryOpen. 2016 ; 巻 5, 番号 6. pp. 508-512.
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