TY - JOUR
T1 - Rapid Osseointegration Bestowed by Carbonate Apatite Coating of Rough Titanium
AU - Shi, Rui
AU - Hayashi, Koichiro
AU - Ishikawa, Kunio
N1 - Funding Information:
This study was supported partially by Japan Agency for Medical Research and Development under Grant No. JP19im0502004 and Japan Society for the Promotion of Science KAKENHI Grant No. JP19K22970. The authors also thank the support from the China Scholarship Council (CSC, No. 201708050163) to Rui Shi.
Publisher Copyright:
© 2020 The Authors. Published by Wiley-VCH GmbH
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Titanium (Ti) implants that realize rapid osseointegration are required for favorable outcomes. Rough implant surfaces favor osseointegration, hence, coating implants with natural bone mineral, i.e., carbonate apatite (CO3Ap), may be effective for osseointegration. To achieve rapid osseointegration, rough-Ti substrates are coated with CO3Ap (CO3Ap-Ti) and the effects are evaluated in vitro and in vivo. For comparison, rough-Ti without coating (rough-Ti) and calcite-coated rough-Ti (calcite-Ti) substrates are fabricated. The adhesive strengths of calcite and CO3Ap to the substrates are ≈56.6 and ≈76.8 MPa, respectively, being significantly higher than the strength defined in ISO13779-2 (15 MPa). Calcite and CO3Ap coatings significantly promote preosteoblastic MC3T3-E1 cell proliferation. Additionally, the CO3Ap coating promotes higher osteogenic differentiation activity than the calcite coating. Implantation of CO3Ap-Ti into rabbit tibia defects prompts bone maturation, compared to calcite-Ti or rough-Ti implantation. The bone-implant contact percentage with CO3Ap-Ti and calcite-Ti is higher than that with rough-Ti. Consequently, CO3Ap-Ti acquires a robust bond with the host bone at an early stage (4 weeks postimplantation), compared to calcite-Ti or rough-Ti: the CO3Ap-Ti–bone bonding strength is ≈1.9- and ≈5.5-fold higher than that of calcite-Ti and rough-Ti, respectively. Thus, CO3Ap coating of Ti implants effectively achieve rapid osseointegration.
AB - Titanium (Ti) implants that realize rapid osseointegration are required for favorable outcomes. Rough implant surfaces favor osseointegration, hence, coating implants with natural bone mineral, i.e., carbonate apatite (CO3Ap), may be effective for osseointegration. To achieve rapid osseointegration, rough-Ti substrates are coated with CO3Ap (CO3Ap-Ti) and the effects are evaluated in vitro and in vivo. For comparison, rough-Ti without coating (rough-Ti) and calcite-coated rough-Ti (calcite-Ti) substrates are fabricated. The adhesive strengths of calcite and CO3Ap to the substrates are ≈56.6 and ≈76.8 MPa, respectively, being significantly higher than the strength defined in ISO13779-2 (15 MPa). Calcite and CO3Ap coatings significantly promote preosteoblastic MC3T3-E1 cell proliferation. Additionally, the CO3Ap coating promotes higher osteogenic differentiation activity than the calcite coating. Implantation of CO3Ap-Ti into rabbit tibia defects prompts bone maturation, compared to calcite-Ti or rough-Ti implantation. The bone-implant contact percentage with CO3Ap-Ti and calcite-Ti is higher than that with rough-Ti. Consequently, CO3Ap-Ti acquires a robust bond with the host bone at an early stage (4 weeks postimplantation), compared to calcite-Ti or rough-Ti: the CO3Ap-Ti–bone bonding strength is ≈1.9- and ≈5.5-fold higher than that of calcite-Ti and rough-Ti, respectively. Thus, CO3Ap coating of Ti implants effectively achieve rapid osseointegration.
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U2 - 10.1002/admi.202000636
DO - 10.1002/admi.202000636
M3 - Article
AN - SCOPUS:85089017109
SN - 2196-7350
VL - 7
JO - Advanced Materials Interfaces
JF - Advanced Materials Interfaces
IS - 18
M1 - 2000636
ER -