Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles

Masashige Shinkai, Masamichi Kamihira, Hiroyuki Honda, Takeshi Kobayashi

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.

本文言語英語
ページ(範囲)256-259
ページ数4
ジャーナルkagaku kogaku ronbunshu
18
2
DOI
出版ステータス出版済み - 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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