Rapid redistribution of the postsynaptic density protein PSD-Zip45 (Homer 1c) and its differential regulation by NMDA receptors and calcium channels

Shigeo Okabe, Tomoe Urushido, Daijiro Konno, Haruo Okado, Kenji Sobue

研究成果: Contribution to journalArticle査読

104 被引用数 (Scopus)

抄録

PSD-Zip45 (Homer 1c) and PSD-95 are postsynaptic density (PSD) proteins containing distinct protein-interacting motifs. Green fluorescent protein (GFP)-tagged PSD-Zip45 and PSD-95 molecules were targeted to the PSD in hippocampal neurons. We analyzed dynamic behavior of these GFP-tagged PSD proteins by using time-lapse confocal microscopy. In contrast to the less dynamic properties of PSD-95, PSD-Zip45 showed rapid redistribution and a higher steady-state turnover rate. Differential stimulation protocols were found to alter the direction of PSD-Zip45 assembly-disassembly. Transient increases in intracellular Ca2+ by voltage-dependent Ca2+ channel activation induced PSD-Zip45 clustering. In contrast, NMDA receptor-dependent Ca2+ influx resulted in the disassembly of PSD-Zip45 clusters. Thus, neuronal activity differentially redistributes a specific subset of PSD proteins, which are important for localization of both surface receptors and intracellular signaling complexes.

本文言語英語
ページ(範囲)9561-9571
ページ数11
ジャーナルJournal of Neuroscience
21
24
DOI
出版ステータス出版済み - 12 15 2001
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

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